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Western blot analysis was performed on whole cell extracts (30 ug lysate) of Caco-2 (Lane 1), NTERA-2 (Lane 2), THP1 (Lane 3), HEL 92.1.7 (Lane 4), HEL 92.1.7 treated with 1 uM Doxorubicin for 18 hours (Lane 5), HeLa (Lane 6), and HeLa treated with 1 uM Doxorubicin for 18 hours (Lane 7). The blots were probed with Anti-Cyclin A Mouse Monoclonal Antibody (Product# MA511306, 1:100-1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A ~ 52 kDa band corresponding to Cyclin A was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.4, with 4mg/ml BSA, 40% glycerol|
|Contains||0.1% Proclin 300|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000-1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization.
62-6520 was used in western blot to determine the function of C11orf83, a mitochondrial protein.
|Desmurs M,Foti M,Raemy E,Vaz FM,Martinou JC,Bairoch A,Lane L||Molecular and cellular biology (35:1139)||2015|
|Not Applicable||Not Cited||
Relationship between Na(+),K(+)-ATPase and cell attachment.
62-6520 was used in western blot to study the effect of ouabain treatment on cell attachment.
|Contreras RG,Shoshani L,Flores-Maldonado C,Lázaro A,Cereijido M||Journal of cell science (112 ( Pt 23):4223)||1999|
Intestinal and liver changes after chronic ketamine and ketamine plus alcohol treatment.
62-6520 was used in immunohistochemistry - paraffin section to examine effects of long-term chronic ketamine treatment on the intestine and the liver.
|Wong YW,Lam LH,Tang HC,Liang Y,Tan S,Yew DT||Microscopy research and technique (75:1170)||2012|