Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin (Product # 62-6540) at dilutions 1:5,000 (Fig. 1) and 1:20,000 (Fig. 2). A 22 kDa band corresponding to SOD2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product# N200, 1:10,000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.4, with 50% glycerol, 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against solid phase human serum protein|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5,000 - 1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphorylation of zona occludens-2 by protein kinase C epsilon regulates its nuclear exportation.
62-6540 was used in immunocytochemistry to study localization of zona occludens-2
|Chamorro D,Alarcón L,Ponce A,Tapia R,González-Aguilar H,Robles-Flores M,Mejía-Castillo T,Segovia J,Bandala Y,Juaristi E,González-Mariscal L||Molecular biology of the cell (20:4120)||2009|
The tight junction protein ZO-2 has several functional nuclear export signals.
62-6540 was used in immunocytochemistry to study the nuclear export signals of canine ZO-2
|González-Mariscal L,Ponce A,Alarcón L,Jaramillo BE||Experimental cell research (312:3323)||2006|
BAG-1 expression correlates with Bcl-2, p53, differentiation, estrogen and progesterone receptors in invasive breast carcinoma.
62-6540 was used in immunohistochemistry - paraffin section to study the expression of BAG-1, Bcl-2, and p53 in breast cancer patients
|Tang SC,Beck J,Murphy S,Chernenko G,Robb D,Watson P,Khalifa M||Breast cancer research and treatment (84:203)||2004|
|Not Applicable||Not Cited||
Histopathologic and molecular alterations in bronchial epithelium in habitual smokers of marijuana, cocaine, and/or tobacco.
62-6540 was used in immunohistochemistry - paraffin section to determine if the molecular and histopathologic alterations that occur in the lungs of tobacco smokers also occur in the lungs of habitual smokers of marijuana and/or cocaine.
|Barsky SH,Roth MD,Kleerup EC,Simmons M,Tashkin DP||Journal of the National Cancer Institute (90:1198)||1998|
|Not Applicable||Not Cited||
Estrogenic activity of natural and synthetic estrogens in human breast cancer cells in culture.
62-6540 was used in immunocytochemistry to compare the ability of estrogen receptor ligands to bind the receptor and induce signaling.
|Zava DT,Blen M,Duwe G||Environmental health perspectives (105 Suppl 3:637)||1997|