Immunofluorescence analysis of F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 568 conjugate (Product # A11019) was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with mouse primary antibody (1:250 dilution) for 3 hours at room temperature. F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 568 conjugate was used at concentration of 2 µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 568|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and serum|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Newly identified minor phosphorylation site threonine-279 of measles virus nucleoprotein is a prerequisite for nucleocapsid formation.||Sugai A,Sato H,Hagiwara K,Kozuka-Hata H,Oyama M,Yoneda M,Kai C||Journal of virology (88:1140)||2014|
|Not Applicable||Not Cited||Protein kinase D regulates cofilin activity through p21-activated kinase 4.||Spratley SJ,Bastea LI,Döppler H,Mizuno K,Storz P||The Journal of biological chemistry (286:34254)||2011|
|Not Applicable||Not Cited||Super-resolution imaging with small organic fluorophores.||Heilemann M,van de Linde S,Mukherjee A,Sauer M||Angewandte Chemie (International ed. in English) (48:6903)||2009|
|Not Applicable||Not Cited||Hepatic progenitor cells in human fetal liver express the oval cell marker Thy-1.||Masson NM,Currie IS,Terrace JD,Garden OJ,Parks RW,Ross JA||American journal of physiology. Gastrointestinal and liver physiology (291:G45)||2006|
|Not Applicable||Not Cited||
The coxsackie B virus and adenovirus receptor resides in a distinct membrane microdomain.
A-11019 was used in flow cytometry to elucidate the cell membrane microdomain of CAR.
|Ashbourne Excoffon KJ,Moninger T,Zabner J||Journal of virology (77:2559)||2003|
|Not Applicable||Not Cited||Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor.||Croze E,Usacheva A,Asarnow D,Minshall RD,Perez HD,Colamonici O||Journal of immunology (Baltimore, Md. : 1950) (165:5127)||2000|
Fall in density, but not number of myenteric neurons and circular muscle nerve fibres in guinea-pig colon with ageing.
A-11019 was used in immunohistochemistry to examine age-associated changes in the guinea-pig ileum
|Peck CJ,Samsuria SD,Harrington AM,King SK,Hutson JM,Southwell BR||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (21:1075)||2009|