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Muntjac cells were treated with 10 µM EdU (Cat. No. A10044) for 45 min; cells were then fixed and permeabilized, and EdU that had been incorporated into newly synthesized DNA was detected using the far red–fluorescent Click-iT™ EdU Alexa Fluor® 647 High-Throughput (HCS) Assay (Cat. No. A10208), utilizing the technical tip for converting the HCS assay to conventional fluorescence microscopy. Tubulin was labeled with an anti-α-tubulin antibody (Cat. No. A11126) and visualized with Alexa Fluor® 350 Goat Anti–Mouse IgG (Cat. No. A11045, A21049). The Golgi complex was stained with green-fluorescent Alexa Fluor® 488 conjugate of lectin HPA from Helix pomatia (edible snail) (Cat. No. L11271), and peroxisomes were labeled with an anti-peroxisome antibody and visualized with orange-fluorescent Alexa Fluor® 555 Donkey Anti–Rabbit IgG (Cat. No. A31572).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 350|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The analysis of neurovascular remodeling in entorhino-hippocampal organotypic slice cultures.
A-11045 was used in western blot to describe a slice culture method to study changes to the neurons and blood brain barrier.
|Chip S,Zhu X,Kapfhammer JP||Journal of visualized experiments : JoVE (null:null)||2014|
|Not Applicable||Not Cited||Microinjected antibodies interfere with protein nucleocytoplasmic shuttling by distinct molecular mechanisms.||Marg A,Meyer T,Vigneron M,Vinkemeier U||Cytometry. Part A : the journal of the International Society for Analytical Cytology (73A:1128)||2008|
|Not Applicable||Not Cited||Asymmetric T lymphocyte division in the initiation of adaptive immune responses.||Chang JT,Palanivel VR,Kinjyo I,Schambach F,Intlekofer AM,Banerjee A,Longworth SA,Vinup KE,Mrass P,Oliaro J,Killeen N,Orange JS,Russell SM,Weninger W,Reiner SL||Science (New York, N.Y.) (315:1687)||2007|
|Not Applicable||Not Cited||NIPA1(SPG6), the basis for autosomal dominant form of hereditary spastic paraplegia, encodes a functional Mg2+ transporter.||Goytain A,Hines RM,El-Husseini A,Quamme GA||The Journal of biological chemistry (282:8060)||2007|
|Not Applicable||Not Cited||Glycerin suppression of fluorescence self-quenching and improvement of heterogeneous fluoroimmunoassay sensitivity.||Petrou PS,Mastichiadis C,Christofidis I,Kakabakos SE||Analytical chemistry (79:647)||2007|
|Not Applicable||Not Cited||The armadillo protein p0071 regulates Rho signalling during cytokinesis.||Wolf A,Keil R,Götzl O,Mun A,Schwarze K,Lederer M,Hüttelmaier S,Hatzfeld M||Nature cell biology (8:1432)||2006|
|Not Applicable||Not Cited||The Salmonella effector protein PipB2 is a linker for kinesin-1.||Henry T,Couillault C,Rockenfeller P,Boucrot E,Dumont A,Schroeder N,Hermant A,Knodler LA,Lecine P,Steele-Mortimer O,Borg JP,Gorvel JP,Méresse S||Proceedings of the National Academy of Sciences of the United States of America (103:13497)||2006|
|Not Applicable||Not Cited||Formation of GW bodies is a consequence of microRNA genesis.||Pauley KM,Eystathioy T,Jakymiw A,Hamel JC,Fritzler MJ,Chan EK||EMBO reports (7:904)||2006|
|Not Applicable||Not Cited||Localized recruitment and activation of RhoA underlies dendritic spine morphology in a glutamate receptor-dependent manner.||Schubert V,Da Silva JS,Dotti CG||The Journal of cell biology (172:453)||2006|
|Not Applicable||Not Cited||Centrosome localization determines neuronal polarity.||de Anda FC,Pollarolo G,Da Silva JS,Camoletto PG,Feiguin F,Dotti CG||Nature (436:704)||2005|
|Not Applicable||Not Cited||DNA replication origin plasticity and perturbed fork progression in human inverted repeats.||Lebofsky R,Bensimon A||Molecular and cellular biology (25:6789)||2005|
|Not Applicable||Not Cited||Initiation of embryonic cardiac pacemaker activity by inositol 1,4,5-trisphosphate-dependent calcium signaling.||Méry A,Aimond F,Ménard C,Mikoshiba K,Michalak M,Pucéat M||Molecular biology of the cell (16:2414)||2005|
|Not Applicable||Not Cited||ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains.||Heilbronn R,Engstler M,Weger S,Krahn A,Schetter C,Boshart M||Nucleic acids research (31:6206)||2003|
|Not Applicable||Not Cited||Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation.||Ashman R,Crimeen-Irwin B,Ludford-Menting M,Russell S,Gu M||Scanning (25:116)||2003|
|Not Applicable||Not Cited||Protein kinase C isoforms are translocated to microtubules in neurons.||Nakhost A,Kabir N,Forscher P,Sossin WS||The Journal of biological chemistry (277:40633)||2002|
|Not Applicable||Not Cited||TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2.||Hayes S,Chawla A,Corvera S||The Journal of cell biology (158:1239)||2002|
|Not Applicable||Not Cited||The Rho-associated protein kinase p160ROCK is required for centrosome positioning.||Chevrier V,Piel M,Collomb N,Saoudi Y,Frank R,Paintrand M,Narumiya S,Bornens M,Job D||The Journal of cell biology (157:807)||2002|
|Not Applicable||Not Cited||Structure-function analysis of NADE: identification of regions that mediate nerve growth factor-induced apoptosis.||Mukai J,Shoji S,Kimura MT,Okubo S,Sano H,Suvanto P,Li Y,Irie S,Sato TA||The Journal of biological chemistry (277:13973)||2002|
|Not Applicable||Not Cited||PYK2 as a mediator of endothelin-1/G alpha 11 signaling to GLUT4 glucose transporters.||Park JG,Bose A,Leszyk J,Czech MP||The Journal of biological chemistry (276:47751)||2001|
|Not Applicable||Not Cited||Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium.||Berman-Frank I,Lundgren P,Chen YB,Küpper H,Kolber Z,Bergman B,Falkowski P||Science (New York, N.Y.) (294:1534)||2001|
|Not Applicable||Not Cited||Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.||Dan C,Kelly A,Bernard O,Minden A||The Journal of biological chemistry (276:32115)||2001|
|Not Applicable||Not Cited||Dynamic modulation of cytoskeletal proteins linking integrins to signaling complexes in spreading cells. Role of skelemin in initial integrin-induced spreading.||Reddy KB,Bialkowska K,Fox JE||The Journal of biological chemistry (276:28300)||2001|
|Not Applicable||Not Cited||Heterologous inhibition of G protein-coupled receptor endocytosis mediated by receptor-specific trafficking of beta-arrestins.||Klein U,Müller C,Chu P,Birnbaumer M,von Zastrow M||The Journal of biological chemistry (276:17442)||2001|
|Not Applicable||Not Cited||Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking.||Phillips SA,Barr VA,Haft DH,Taylor SI,Haft CR||The Journal of biological chemistry (276:5074)||2001|
|Not Applicable||Not Cited||Conditional expression of a truncated fragment of nonmuscle myosin II-A alters cell shape but not cytokinesis in HeLa cells.||Wei Q,Adelstein RS||Molecular biology of the cell (11:3617)||2000|
|Not Applicable||Not Cited||Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton.||Dong LQ,Landa LR,Wick MJ,Zhu L,Mukai H,Ono Y,Liu F||Proceedings of the National Academy of Sciences of the United States of America (97:5089)||2000|
|Not Applicable||Not Cited||The TSC1 tumour suppressor hamartin regulates cell adhesion through ERM proteins and the GTPase Rho.||Lamb RF,Roy C,Diefenbach TJ,Vinters HV,Johnson MW,Jay DG,Hall A||Nature cell biology (2:281)||2000|
|Not Applicable||Not Cited||Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation.||Kessels MM,Engqvist-Goldstein AE,Drubin DG||Molecular biology of the cell (11:393)||2000|
|Not Applicable||Not Cited||Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.||Panchuk-Voloshina N,Haugland RP,Bishop-Stewart J,Bhalgat MK,Millard PJ,Mao F,Leung WY,Haugland RP||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (47:1179)||1999|