Immunofluorescence analysis of F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 633 (Product # A21053) was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with mouse primary antibody (1:250 dilution) for 3 hours at room temperature. F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 633 was used at concentration of 4µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 633|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and serum|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||4 µg/ml|
|Immunofluorescence (IF)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Down-regulation of Decapping Protein 2 mediates chronic nicotine exposure-induced locomotor hyperactivity in Drosophila.
A-21053 was used in immunohistochemistry to report a role for the mRNA decapping pathway in developing locomotor hyperactivity in response to chronic nicotine exposure
|Ren J,Sun J,Zhang Y,Liu T,Ren Q,Li Y,Guo A||PloS one (7:null)||2013|
|Not Applicable||Not Cited||Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae.||Mironov AA,Beznoussenko GV,Nicoziani P,Martella O,Trucco A,Kweon HS,Di Giandomenico D,Polishchuk RS,Fusella A,Lupetti P,Berger EG,Geerts WJ,Koster AJ,Burger KN,Luini A||The Journal of cell biology (155:1225)||2001|
|Not Applicable||Not Cited||Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin.||Moser TL,Kenan DJ,Ashley TA,Roy JA,Goodman MD,Misra UK,Cheek DJ,Pizzo SV||Proceedings of the National Academy of Sciences of the United States of America (98:6656)||2001|