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Goat anti-Mouse IgG (H+L) Alexa Fluor 680 Secondary Antibody in WB Western blot analysis of total Cadherin and N-Cadherin was performed by loading 2ul SeeBlue® Plus2 Prestained Protein Ladder (Product # LC5925), 50ug of MDCK cell lysates and 10ug mouse heart lysate per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Total cadherin was detected using a rabbit antibody (Product # 71-7100) and N-Cadherin was detected using a mouse antibody (Product #33-3900), both at a concentration of 1ug/ml in blocking buffer overnight at 4°C on a rocking platform. The blot was then incubated with goat anti-rabbit IgG-Alexa Fluor 790 secondary antibody (Product # A11369) and goat anti-mouse IgG-Alexa Fluor 680 secondary antibody (Product #A21058) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Images generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 680|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:250-1:500|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Western Blot (WB)||1:5,000- 1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Neuronal activity enhances tau propagation and tau pathology in vivo.
A-21058 was used in western blot to characterize enhanced tau propagation and tau pathology in vivo by neuronal activity
|Wu JW,Hussaini SA,Bastille IM,Rodriguez GA,Mrejeru A,Rilett K,Sanders DW,Cook C,Fu H,Boonen RA,Herman M,Nahmani E,Emrani S,Figueroa YH,Diamond MI,Clelland CL,Wray S,Duff KE||Nature neuroscience (19:1085)||2016|
Assembly Dynamics and Stoichiometry of the Apoptosis Signal-regulating Kinase (ASK) Signalosome in Response to Electrophile Stress.
A-21058 was used in western blot to characterize the response to electrophile stress by assembly stoichiometry and dynamics of the apoptosis signal-regulating kinase (ASK) signalosome
|Federspiel JD,Codreanu SG,Palubinsky AM,Winland AJ,Betanzos CM,McLaughlin B,Liebler DC||Molecular and cellular proteomics : MCP (15:1947)||2016|
|Not Applicable||Not Cited||4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.||Baddeley D,Crossman D,Rossberger S,Cheyne JE,Montgomery JM,Jayasinghe ID,Cremer C,Cannell MB,Soeller C||PloS one (6:null)||2011|
|Not Applicable||Not Cited||Nucleotide excision repair-induced H2A ubiquitination is dependent on MDC1 and RNF8 and reveals a universal DNA damage response.||Marteijn JA,Bekker-Jensen S,Mailand N,Lans H,Schwertman P,Gourdin AM,Dantuma NP,Lukas J,Vermeulen W||The Journal of cell biology (186:835)||2009|
|Not Applicable||Not Cited||Fibroblast growth factor receptor 1 is a key regulator of early adipogenic events in human preadipocytes.||Widberg CH,Newell FS,Bachmann AW,Ramnoruth SN,Spelta MC,Whitehead JP,Hutley LJ,Prins JB||American journal of physiology. Endocrinology and metabolism (296:E121)||2009|
|Not Applicable||Not Cited||alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering.||Dobbins GC,Luo S,Yang Z,Xiong WC,Mei L||Molecular brain (1:null)||2008|
|Not Applicable||Not Cited||Quantitative evaluation of signaling events in Drosophila S2 cells.||Bond D,Primrose DA,Foley E||Biological procedures online (10:20)||2008|
|Not Applicable||Not Cited||Tumor necrosis factor receptor 2 signaling induces selective c-IAP1-dependent ASK1 ubiquitination and terminates mitogen-activated protein kinase signaling.||Zhao Y,Conze DB,Hanover JA,Ashwell JD||The Journal of biological chemistry (282:7777)||2007|
|Not Applicable||Not Cited||Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.||Silva E,Gomes P,Soares-da-Silva P||The Journal of membrane biology (212:163)||2007|
|Not Applicable||Not Cited||Three mammalian lipins act as phosphatidate phosphatases with distinct tissue expression patterns.||Donkor J,Sariahmetoglu M,Dewald J,Brindley DN,Reue K||The Journal of biological chemistry (282:3450)||2007|
|Not Applicable||Not Cited||Identification of cysteines involved in S-nitrosylation, S-glutathionylation, and oxidation to disulfides in ryanodine receptor type 1.||Aracena-Parks P,Goonasekera SA,Gilman CP,Dirksen RT,Hidalgo C,Hamilton SL||The Journal of biological chemistry (281:40354)||2006|
|Not Applicable||Not Cited||Cell-surface heparan sulfate proteoglycans are essential components of the unconventional export machinery of FGF-2.||Zehe C,Engling A,Wegehingel S,Schäfer T,Nickel W||Proceedings of the National Academy of Sciences of the United States of America (103:15479)||2006|
|Not Applicable||Not Cited||Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon.||Stefansson B,Brautigan DL||The Journal of biological chemistry (281:22624)||2006|
|Not Applicable||Not Cited||Negative regulation of Fc epsilonRI-mediated signaling and mast cell function by the adaptor protein LAX.||Zhu M,Rhee I,Liu Y,Zhang W||The Journal of biological chemistry (281:18408)||2006|
|Not Applicable||Not Cited||A novel cell-based assay for G-protein-coupled receptor-mediated cyclic adenosine monophosphate response element binding protein phosphorylation.||Selkirk JV,Nottebaum LM,Ford IC,Santos M,Malany S,Foster AC,Lechner SM||Journal of biomolecular screening (11:351)||2006|
|Not Applicable||Not Cited||TLR1- and TLR6-independent recognition of bacterial lipopeptides.||Buwitt-Beckmann U,Heine H,Wiesmüller KH,Jung G,Brock R,Akira S,Ulmer AJ||The Journal of biological chemistry (281:9049)||2006|
|Not Applicable||Not Cited||Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids.||Kono M,Dreier JL,Ellis JM,Allende ML,Kalkofen DN,Sanders KM,Bielawski J,Bielawska A,Hannun YA,Proia RL||The Journal of biological chemistry (281:7324)||2006|
|Not Applicable||Not Cited||Pharmacological promotion of inclusion formation: a therapeutic approach for Huntington's and Parkinson's diseases.||Bodner RA,Outeiro TF,Altmann S,Maxwell MM,Cho SH,Hyman BT,McLean PJ,Young AB,Housman DE,Kazantsev AG||Proceedings of the National Academy of Sciences of the United States of America (103:4246)||2006|
|Not Applicable||Not Cited||Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II.||Foulquier F,Vasile E,Schollen E,Callewaert N,Raemaekers T,Quelhas D,Jaeken J,Mills P,Winchester B,Krieger M,Annaert W,Matthijs G||Proceedings of the National Academy of Sciences of the United States of America (103:3764)||2006|
|Not Applicable||Not Cited||Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging.||Yasuda R,Harvey CD,Zhong H,Sobczyk A,van Aelst L,Svoboda K||Nature neuroscience (9:283)||2006|
|Not Applicable||Not Cited||The glycosphingolipid globotriaosylceramide in the metastatic transformation of colon cancer.||Kovbasnjuk O,Mourtazina R,Baibakov B,Wang T,Elowsky C,Choti MA,Kane A,Donowitz M||Proceedings of the National Academy of Sciences of the United States of America (102:19087)||2005|
|Not Applicable||Not Cited||The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin.||Qin Y,Capaldo C,Gumbiner BM,Macara IG||The Journal of cell biology (171:1061)||2005|
|Not Applicable||Not Cited||The membrane-bound histidine acid phosphatase TbMBAP1 is essential for endocytosis and membrane recycling in Trypanosoma brucei.||Engstler M,Weise F,Bopp K,Grünfelder CG,Günzel M,Heddergott N,Overath P||Journal of cell science (118:2105)||2005|
|Not Applicable||Not Cited||Visualization and quantitation of peroxisomes using fluorescent nanocrystals: treatment of rats and monkeys with fibrates and detection in the liver.||Colton HM,Falls JG,Ni H,Kwanyuen P,Creech D,McNeil E,Casey WM,Hamilton G,Cariello NF||Toxicological sciences : an official journal of the Society of Toxicology (80:183)||2004|
|Not Applicable||Not Cited||Ras regulates assembly of mitogenic signalling complexes through the effector protein IMP.||Matheny SA,Chen C,Kortum RL,Razidlo GL,Lewis RE,White MA||Nature (427:256)||2004|