Flow cytometry analysis of Chicken anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A21201) was performed using K-562 cells stained with alpha Tubulin Mouse Monoclonal Antibody (A11126). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with alpha Tubulin antibody or with mouse isotype control at 3-5 ug/million cells in 2.5% BSA and incubated for 2 hours at room temperature. The cells were then labeled with Chicken anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A21201) at a dilution of 1:500 for 1 hour at room temperature. A representative 10,000 cells were acquired and analyzed for each sample using the Attune® NxT Acoustic Focusing Cytometer. Panels a, b and c represent cells stained with the secondary antibody alone, isotype control and alpha Tubulin Monoclonal Antibody respectively. Median fluorescence intensity from the three samples is compared in panel d.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human and rabbit IgG prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:500|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A MAP6-related protein is present in protozoa and is involved in flagellum motility.
A-21201 was used in immunocytochemistry to identify TbSAXO as the first MAP6-related protein identified in Trypanosoma brucei
|Dacheux D,Landrein N,Thonnus M,Gilbert G,Sahin A,Wodrich H,Robinson DR,Bonhivers M||PloS one (7:null)||2012|
|Not Applicable||Not Cited||
Extracellular matrix-induced transforming growth factor-beta receptor signaling dynamics.
A-21201 was used in immunocytochemistry to elucidate TGF-beta receptor dynamics and consequential signaling
|Garamszegi N,Garamszegi SP,Samavarchi-Tehrani P,Walford E,Schneiderbauer MM,Wrana JL,Scully SP||Oncogene (29:2368)||2010|
|Not Applicable||Not Cited||
Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues.
A-21201 was used in immunohistochemistry to develop three-dimensional muscle tissue architectures in vitro
|Bian W,Liau B,Badie N,Bursac N||Nature protocols (4:1522)||2009|
|Not Applicable||Not Cited||Deficient ghrelin receptor-mediated signaling compromises thymic stromal cell microenvironment by accelerating thymic adiposity.||Youm YH,Yang H,Sun Y,Smith RG,Manley NR,Vandanmagsar B,Dixit VD||The Journal of biological chemistry (284:7068)||2009|
|Not Applicable||Not Cited||Filamin B mediates ICAM-1-driven leukocyte transendothelial migration.||Kanters E,van Rijssel J,Hensbergen PJ,Hondius D,Mul FP,Deelder AM,Sonnenberg A,van Buul JD,Hordijk PL||The Journal of biological chemistry (283:31830)||2008|
|Not Applicable||Not Cited||Aberrant expression of ID2, a suppressor of B-cell-specific gene expression, in Hodgkin's lymphoma.||Renné C,Martin-Subero JI,Eickernjäger M,Hansmann ML,Küppers R,Siebert R,Bräuninger A||The American journal of pathology (169:655)||2006|
|Not Applicable||Not Cited||A novel member of the IkappaB family, human IkappaB-zeta, inhibits transactivation of p65 and its DNA binding.||Totzke G,Essmann F,Pohlmann S,Lindenblatt C,Jänicke RU,Schulze-Osthoff K||The Journal of biological chemistry (281:12645)||2006|
|Not Applicable||Not Cited||EphB receptors regulate dendritic spine morphogenesis through the recruitment/phosphorylation of focal adhesion kinase and RhoA activation.||Moeller ML,Shi Y,Reichardt LF,Ethell IM||The Journal of biological chemistry (281:1587)||2006|
|Not Applicable||Not Cited||Specific recruitment of human cohesin to laser-induced DNA damage.||Kim JS,Krasieva TB,LaMorte V,Taylor AM,Yokomori K||The Journal of biological chemistry (277:45149)||2002|