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A comparison of the photobleaching rates of APC and Cy5 conjugates. The micro172tubules of bovine pulmonary artery endothelial cells were stained with mouse anti-a-tubulin antibody (Cat. no. A11126) in combination with goat anti-mouse IgG labeled antibody with either crosslinked APC (Cat. no. A865, top series) or the Cy5 dye (bottom series). The samples were exposed to continuous illumination, and the images were acquired at 30-second intervals with a Quantex cooled CCD camera (Photometrics) using filter sets appropriate for both APC and Cy5 dye.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/ml|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 6 publications below|
The allophycocyanin (APC), crosslinked, goat anti-mouse IgG is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. Because this phycobiliprotein is optimally excited at 633 nm but can also be excited at 594 nm, it can be used with samples simultaneously labeled with Texas Red dye or Alexa Fluor 594 dye without a second excitation source. Flourescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope. APC can be used in flow cytometry and imaging applications and is more photostable than Cy5 conjugates.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly.||Ma Y,Yates J,Liang Y,Lemon SM,Yi M||Journal of virology (82:7624)||2008|
|Not Applicable||Not Cited||Sensitive fluorescent detection of protein on nylon membranes.||Dubitsky A,DeCollibus D,Ortolano GA||Journal of biochemical and biophysical methods (51:47)||2002|
|Not Applicable||Not Cited||Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.||Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM||The Journal of biological chemistry (277:2287)||2002|
|Not Applicable||Not Cited||Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3.||McDonald PH,Chow CW,Miller WE,Laporte SA,Field ME,Lin FT,Davis RJ,Lefkowitz RJ||Science (New York, N.Y.) (290:1574)||2000|
|Not Applicable||Not Cited||Simultaneous five-six color multiparameter analysis.||Pennline KJ||Methods in molecular biology (Clifton, N.J.) (91:255)||1998|
|Not Applicable||Not Cited||Evaluation of fluorochromes and excitation sources for immunofluorescence in water samples.||Vesey G,Deere D,Gauci MR,Griffiths KR,Williams KL,Veal DA||Cytometry (29:147)||1997|