|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/ml|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioengineered uterine tissue supports pregnancy in a rat model.
A10521 was used in immunohistochemistry - frozen section to study pregnancy in a rat model using bioengineered uterine tissue
|Hellström M,Moreno-Moya JM,Bandstein S,Bom E,Akouri RR,Miyazaki K,Maruyama T,Brännström M||Fertility and sterility (106:487)||2016|
Four Individually Identified Paired Dopamine Neurons Signal Reward in Larval Drosophila.
A10521 was used in immunohistochemistry to determine the function of different dopaminergic neurons using larval Drosophila
|Rohwedder A,Wenz NL,Stehle B,Huser A,Yamagata N,Zlatic M,Truman JW,Tanimoto H,Saumweber T,Gerber B,Thum AS||Current biology : CB (26:661)||2016|
Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae.
A10521 was used in immunohistochemistry to test if Drosophila neuropeptide F neurons are involved in classical olfactory conditioning
|Rohwedder A,Selcho M,Chassot B,Thum AS||The Journal of comparative neurology (523:2637)||2015|
Eye-independent, light-activated chromatophore expansion (LACE) and expression of phototransduction genes in the skin of Octopus bimaculoides.
A10521 was used in immunocytochemistry to elucidate how octopus skin senses light
|Ramirez MD,Oakley TH||The Journal of experimental biology (218:1513)||2015|
Prostaglandin E2 (PGE2) exerts biphasic effects on human tendon stem cells.
A10521 was used in immunocytochemistry to determine the biphasic effects of PGE2 on human tendon stem/progenitor cells.
|Zhang J,Wang JH||PloS one (9:null)||2014|