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FluoCells prepared slide #2 (Prod # F14781), which shows bovine pulmonary artery endothelial cells (BPAEC) that have been stained with an anti-β-tubulin mouse monoclonal antibody in conjunction with BODIPY FL goat anti-mouse IgG (Prod # B2752) for labeling microtubules, Texas Red-X phalloidin (Prod # T7471) for labeling F-actin and DAPI (Prod # D1306, D3571, D21490) for labeling nuclei. This multiple-exposure image was acquired using bandpass optical filter sets appropriate for DAPI, fluorescein and Texas Red dye.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5, with 1.5% BSA|
|Storage Conditions||-20° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 21 publications below|
Storage and reconstitution: reconstitute using 0.5 mL deionized water. The product can be stored up to 2 weeks at 2-8°C. For longer storage, divide into single-use aliquots and freeze at or below -20°C. Frozen aliquots are stable for at least 6 months.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||A pool of extramitochondrial frataxin that promotes cell survival.||Condò I,Ventura N,Malisan F,Tomassini B,Testi R||The Journal of biological chemistry (281:16750)||2006|
|Not Applicable||Not Cited||Shear stress-induced endothelial cell polarization is mediated by Rho and Rac but not Cdc42 or PI 3-kinases.||Wojciak-Stothard B,Ridley AJ||The Journal of cell biology (161:429)||2003|
|Not Applicable||Not Cited||Localization of 5-HT(2A) receptors on dopamine cells in subnuclei of the midbrain A10 cell group.||Nocjar C,Roth BL,Pehek EA||Neuroscience (111:163)||2002|
|Not Applicable||Not Cited||Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2.||Choi EK,Zaidi NF,Miller JS,Crowley AC,Merriam DE,Lilliehook C,Buxbaum JD,Wasco W||The Journal of biological chemistry (276:19197)||2001|
|Not Applicable||Not Cited||The dynamin-dependent, arrestin-independent internalization of 5-hydroxytryptamine 2A (5-HT2A) serotonin receptors reveals differential sorting of arrestins and 5-HT2A receptors during endocytosis.||Bhatnagar A,Willins DL,Gray JA,Woods J,Benovic JL,Roth BL||The Journal of biological chemistry (276:8269)||2001|
|Not Applicable||Not Cited||Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases.||Lavoie JN,Champagne C,Gingras MC,Robert A||The Journal of cell biology (150:1037)||2000|
|Not Applicable||Not Cited||Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity.||Eger A,Stockinger A,Schaffhauser B,Beug H,Foisner R||The Journal of cell biology (148:173)||2000|
|Not Applicable||Not Cited||H-Ras activation promotes cytoplasmic accumulation and phosphoinositide 3-OH kinase association of beta-catenin in epidermal keratinocytes.||Espada J,Pérez-Moreno M,Braga VM,Rodriguez-Viciana P,Cano A||The Journal of cell biology (146:967)||1999|
|Not Applicable||Not Cited||Identification of an alpha(3)beta(1) integrin recognition sequence in thrombospondin-1.||Krutzsch HC,Choe BJ,Sipes JM,Guo Nh,Roberts DD||The Journal of biological chemistry (274:24080)||1999|
|Not Applicable||Not Cited||Pro-adhesive and chemotactic activities of thrombospondin-1 for breast carcinoma cells are mediated by alpha3beta1 integrin and regulated by insulin-like growth factor-1 and CD98.||Chandrasekaran S,Guo NH,Rodrigues RG,Kaiser J,Roberts DD||The Journal of biological chemistry (274:11408)||1999|
|Not Applicable||Not Cited||The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells.||Glaven JA,Whitehead I,Bagrodia S,Kay R,Cerione RA||The Journal of biological chemistry (274:2279)||1999|
|Not Applicable||Not Cited||Blockade of NMDA receptors and apoptotic neurodegeneration in the developing brain.||Ikonomidou C,Bosch F,Miksa M,Bittigau P,Vöckler J,Dikranian K,Tenkova TI,Stefovska V,Turski L,Olney JW||Science (New York, N.Y.) (283:70)||1999|
|Not Applicable||Not Cited||Inhibition of nonspecific binding of fluorescent-labelled antibodies to human eosinophils.||Mahmudi-Azer S,Lacy P,Bablitz B,Moqbel R||Journal of immunological methods (217:113)||1998|
|Not Applicable||Not Cited||Cytoskeletal association is important for differential targeting of glucose transporter isoforms in Leishmania.||Snapp EL,Landfear SM||The Journal of cell biology (139:1775)||1997|
|Not Applicable||Not Cited||Molecular characterization of abLIM, a novel actin-binding and double zinc finger protein.||Roof DJ,Hayes A,Adamian M,Chishti AH,Li T||The Journal of cell biology (138:575)||1997|
|Not Applicable||Not Cited||Analysis of mitochondrial morphology and function with novel fixable fluorescent stains.||Poot M,Zhang YZ,Krämer JA,Wells KS,Jones LJ,Hanzel DK,Lugade AG,Singer VL,Haugland RP||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (44:1363)||1996|
|Not Applicable||Not Cited||Mammalian Cdc42 is a brefeldin A-sensitive component of the Golgi apparatus.||Erickson JW,Zhang Cj,Kahn RA,Evans T,Cerione RA||The Journal of biological chemistry (271:26850)||1996|
|Not Applicable||Not Cited||Modulatory actions of dopamine on NMDA receptor-mediated responses are reduced in D1A-deficient mutant mice.||Levine MS,Altemus KL,Cepeda C,Cromwell HC,Crawford C,Ariano MA,Drago J,Sibley DR,Westphal H||The Journal of neuroscience : the official journal of the Society for Neuroscience (16:5870)||1996|
|Not Applicable||Not Cited||How an actin network might cause fountain streaming and nuclear migration in the syncytial Drosophila embryo.||von Dassow G,Schubiger G||The Journal of cell biology (127:1637)||1994|
|Not Applicable||Not Cited||Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes.||Casciola-Rosen LA,Anhalt G,Rosen A||The Journal of experimental medicine (179:1317)||1994|
|Not Applicable||Not Cited||Simultaneous visualization of G- and F-actin in endothelial cells.||Haugland RP,You W,Paragas VB,Wells KS,DuBose DA||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (42:345)||1994|