|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 8 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Activation of endogenous Cdc42 visualized in living cells.||Nalbant P,Hodgson L,Kraynov V,Toutchkine A,Hahn KM||Science (New York, N.Y.) (305:1615)||2004|
|Not Applicable||Not Cited||Mutational analysis of fibrillarin and its mobility in living human cells.||Snaar S,Wiesmeijer K,Jochemsen AG,Tanke HJ,Dirks RW||The Journal of cell biology (151:653)||2000|
|Not Applicable||Not Cited||Multicolor immunofluorescence and flow cytometry utilizing cascade blue to purify murine hematopoietic stem cells from fetal liver and bone marrow.||Donahue CJ,Fennie C,Villacorta R,La H,Lasky LA,Ohneda O||Cytometry (37:60)||1999|
|Not Applicable||Not Cited||Interferon alpha inhibits a Src-mediated pathway necessary for Shigella-induced cytoskeletal rearrangements in epithelial cells.||Duménil G,Olivo JC,Pellegrini S,Fellous M,Sansonetti PJ,Nhieu GT||The Journal of cell biology (143:1003)||1998|
|Not Applicable||Not Cited||Fiber-optic immunosensor for measurement of myoglobin.||Hanbury CM,Miller WG,Harris RB||Clinical chemistry (43:2128)||1997|
|Not Applicable||Not Cited||Evaluation of fluorochromes and excitation sources for immunofluorescence in water samples.||Vesey G,Deere D,Gauci MR,Griffiths KR,Williams KL,Veal DA||Cytometry (29:147)||1997|
|Not Applicable||Not Cited||Bone morphogenetic proteins promote astroglial lineage commitment by mammalian subventricular zone progenitor cells.||Gross RE,Mehler MF,Mabie PC,Zang Z,Santschi L,Kessler JA||Neuron (17:595)||1996|
|Not Applicable||Not Cited||Simultaneous visualization of G- and F-actin in endothelial cells.||Haugland RP,You W,Paragas VB,Wells KS,DuBose DA||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (42:345)||1994|