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Three-color staining of HeLa cells using fluorescent Qdot® nanocrystal conjugates. The intracellular structures in fixed HeLa cells were visualized using a red-fluorescent Qdot® 655 F(ab')2 goat anti-mouse IgG (Q11021MP, Q11022MP) (nuclei), a yellow-fluorescent Qdot® 585 F(ab')2 goat anti-rabbit IgG (Q11411MP) (Golgi), and a green-fluorescent Qdot® 525 streptavidin conjugate (Q10141MP) (microtubules).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 8 publications below|
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||The short splice variant of the gamma 2 subunit acts as an external modulator of GABA(A) receptor function.||Boileau AJ,Pearce RA,Czajkowski C||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:4895)||2010|
|Not Applicable||Not Cited||A technical note on quantum dots for multi-color fluorescence in situ hybridization.||Müller S,Cremer M,Neusser M,Grasser F,Cremer T||Cytogenetic and genome research (124:351)||2009|
|Not Applicable||Not Cited||Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates.||Kingeter LM,Schaefer BC||BMC biotechnology (9:null)||2009|
|Not Applicable||Not Cited||A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection.||Qian M,Cai D,Verhey KJ,Tsai B||PLoS pathogens (5:null)||2009|
|Not Applicable||Not Cited||NSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal Vγ2Vδ2 T-cell expansion.||Chen Y,Shao L,Ali Z,Cai J,Chen ZW||Blood (111:4220)||2008|
|Not Applicable||Not Cited||Characterization of SynCAM surface trafficking using a SynCAM derived ligand with high homophilic binding affinity.||Breillat C,Thoumine O,Choquet D||Biochemical and biophysical research communications (359:655)||2007|
|Not Applicable||Not Cited||4Pi microscopy of quantum dot-labeled cellular structures.||Medda R,Jakobs S,Hell SW,Bewersdorf J||Journal of structural biology (156:517)||2006|
|Not Applicable||Not Cited||Single plasma membrane K+ channel detection by using dual-color quantum dot labeling.||Nechyporuk-Zloy V,Stock C,Schillers H,Oberleithner H,Schwab A||American journal of physiology. Cell physiology (291:C266)||2006|