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Confocal micrograph illustrating sexual reproduction of the ciliate protist, Tetrahymena thermophila, six hours after mating. After fixation and permeabilization, the cytoskeleton was labeled with an anti-tubulin antibody and subsequently visualized with Texas Red®-X goat anti–mouse IgG antibody (Cat. No. T6390). The macro- and micronuclei were stained with SYTOX® Green nucleic acid stain (Cat. No. S7020). Image contributed by David Asai and Amy Walanski, Purdue University.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 13 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.||Abodeely M,DuBois KN,Hehl A,Stefanic S,Sajid M,DeSouza W,Attias M,Engel JC,Hsieh I,Fetter RD,McKerrow JH||Eukaryotic cell (8:1665)||2009|
|Not Applicable||Not Cited||Heterogeneity in macrophage phagocytosis of Staphylococcus aureus strains: high-throughput scanning cytometry-based analysis.||DeLoid GM,Sulahian TH,Imrich A,Kobzik L||PloS one (4:null)||2009|
|Not Applicable||Not Cited||The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain.||Elia AE,Rellos P,Haire LF,Chao JW,Ivins FJ,Hoepker K,Mohammad D,Cantley LC,Smerdon SJ,Yaffe MB||Cell (115:83)||2003|
|Not Applicable||Not Cited||Synthetic compound libraries displayed on the surface of encoded bacteriophage.||Woiwode TF,Haggerty JE,Katz R,Gallop MA,Barrett RW,Dower WJ,Cwirla SE||Chemistry & biology (10:847)||2003|
|Not Applicable||Not Cited||Glucose 6-phosphate produced by gluconeogenesis and by glucokinase is equally effective in activating hepatic glycogen synthase.||Gomis RR,Favre C,García-Rocha M,Fernández-Novell JM,Ferrer JC,Guinovart JJ||The Journal of biological chemistry (278:9740)||2003|
|Not Applicable||Not Cited||A key role of starburst amacrine cells in originating retinal directional selectivity and optokinetic eye movement.||Yoshida K,Watanabe D,Ishikane H,Tachibana M,Pastan I,Nakanishi S||Neuron (30:771)||2001|
|Not Applicable||Not Cited||The effect of Golgi depletion on exocytic transport.||Pelletier L,Jokitalo E,Warren G||Nature cell biology (2:840)||2000|
|Not Applicable||Not Cited||The amino-terminal domain of the golgi protein giantin interacts directly with the vesicle-tethering protein p115.||Lesa GM,Seemann J,Shorter J,Vandekerckhove J,Warren G||The Journal of biological chemistry (275:2831)||2000|
|Not Applicable||Not Cited||Regulation of epithelial Na(+) channels by actin in planar lipid bilayers and in the Xenopus oocyte expression system.||Jovov B,Tousson A,Ji HL,Keeton D,Shlyonsky V,Ripoll PJ,Fuller CM,Benos DJ||The Journal of biological chemistry (274:37845)||1999|
|Not Applicable||Not Cited||Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor.||Janes PW,Ley SC,Magee AI||The Journal of cell biology (147:447)||1999|
|Not Applicable||Not Cited||A candidate target for G protein action in brain.||Chen LT,Gilman AG,Kozasa T||The Journal of biological chemistry (274:26931)||1999|
|Not Applicable||Not Cited||An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle.||Shima DT,Cabrera-Poch N,Pepperkok R,Warren G||The Journal of cell biology (141:955)||1998|
|Not Applicable||Not Cited||Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras.||Olson KR,McIntosh JR,Olmsted JB||The Journal of cell biology (130:639)||1995|