|Flow Cytometry (Flow)||1:100 (10 µl for 10^6 cells in 100 µl)|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Purified mouse IgG|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
SA1-25258 detects mouse B lymphocytes, mouse IgM and mouse IgG monoclonal antibodies, and mouse Ig kappa chains.
SA1-25258 has been successfully used in flow cytometry procedures. Use 10 µl of the suggested working dilution to label 10^ 6 cells in 100 µl.
The SA1-25258 immunogen is purified mouse IgG.
Store at 4°C short term. For extended periods, store at -20°C, avoiding freeze/thaw cycles.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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