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Human prostate tissue with adenocarcinoma imaged using an Alexa Fluor 488 FluoroNanogold conjugate. Tissues were labeled with an anti-cytokeratin antibody, followed by staining with the Alexa Fluor 488 FluoroNanogold Fab' fragment of goat anti–mouse IgG antibody (Cat. no. A24920). The fluorescence image in the left panel was taken with filters appropriate for fluorescein. The right panel shows a bright-field image of the same system, processed with the LI Silver (LIS) Enhancement Kit (Cat. no. L24919) to visualize the gold distribution. Image courtesy of Nanoprobes, Inc.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Conjugate||Alexa Fluor® 488-FluoroNanogold|
|Storage buffer||PBS, pH 7.4, with 0.1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Immunomicroscopy (IM)||0.2-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Alexa Fluor 488 FluoroNanogold Fab' fragment of goat anti-mouse IgG is covalently conjugated with uniform, 1.4 nm-diameter gold clusters and our exceptionally bright, photostable Alexa Fluor 488 dye (which is spectrally similar to fluorescein). FluoroNanogold conjugates can be used as probes in fluorescence microscopy and, when used with our LI Silver Enhancement Kit (Cat. no. L-24919), in immunoblotting, light microscopy or electron microscopy.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.