Western blot detection of mouse IgG in 100ng per lane of (lane 1) mouse IgG compared to a control with no antibody (lane 2) using a mouse IgG polyclonal antibody (Product # PA1-28555) at a dilution of 1:1000 overnight at 4°C. Secondary detection was performed using a DyLight 800 goat secondary antibody at 1:20,000 for 30 min at room temperature. Samples were blocked for 30 min at room temperature. Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa for mouse IgG.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse IgG, whole molecule|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000-1:5000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000-1:5000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28555 detects Mouse IgG from mouse samples.
PA1-28555 has been successfully used in immunodiffusion, immunohistochemistry (frozen tissue and paraffin), ELISA, immunoprecipitation and western blot applications.
The PA1-28555 immunogen is Mouse IgG, whole molecule.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.