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|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Mouse IgG whole molecule|
|Storage buffer||0.02M potassium phosphate, pH 7.4, with 10mg/ml BSA, 0.15 NaCl|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1500-1:7500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1500-1:7500|
|Western Blot (WB)||1:3000-1:15,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28568 detects Mouse IgG from mouse samples.
PA1-28568 has been successfully used in ELISA, immunomicroscopy, immunohistochemistry (frozen tissue), Western blot, immunohistochemistry (paraffin tissue) and Dot Blot applications.
The PA1-28568 immunogen is Mouse IgG whole molecule.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.