Western blot analysis of rat liver and fish ventricle lysates using Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (MA1-10891). Whole tissue extracts from rat liver (20µg) and Chaenocephalus aceratus and Notothenia coriiceps ventricles (200µg) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Following transfer, membranes were blocked with 5% milk in PBST and probed with Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product #MA1-10891) at 1:2000 dilution followed by donkey anti-mouse IgG secondary antibody (Product # PA1-28748) at 1:5000 dilution and a chemiluminescent substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Mouse|
|Host / Isotype||Donkey / IgG|
|Immunogen||Full length native mouse IgG (purified)|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28748 detects Mouse IgG from mouse samples. Minimum cross reactivity with bovine, chicken, goat, guinea pig, hamster, equine, rat, and ovine samples.
PA1-28748 has been successfully used in ELISA, immunohistochemistry, and Western blot applications.
The PA1-28748 immunogen is full length native mouse IgG (purified)
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.