HeLa cells were transduced with CellLight® Nucleus-GFP (C10602), grown over night. Following fixation, permeabilization and block using the Image-iT Fixation/Permeabilization Kit (R37602), mMitochondria are labeled with mouse anti-ATP synthase subunit IF1 monoclonal antibody (A21355) and detected with ReadyProbes® Alexa Fluor® 594 Goat Anti-Mouse IgG Antibody (R37121). Nuclei were stained with NucBlue® Fixed cell stain (R37606). Images were acquired on the EVOS® FL Auto imaging system using a 20x objective (AMEP4624).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS with 0.1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Spatial distribution of DARPP-32 in dendritic spines.||Blom H,Rönnlund D,Scott L,Westin L,Widengren J,Aperia A,Brismar H||PloS one (8:null)||2013|