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Product Details
PA5-33182
Applications
Tested Dilution
Flow Cytometry (Flow)
Neat
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Cocktail
Type
Isotype Control
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
purified
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2550599
Product Specific Information
PA5-33182 is suitable for use a negative control for measurement of non-specific binding of mouse monoclonals of isotype IgG1 in rat tissue in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. This antibody contains a monoclonal mixture of FITC-conjugated Mouse IgG1 RPE-conjugated Mouse IgG1.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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