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Product Details
PA5-33203
| Applications | Tested Dilution |
|---|---|
Flow Cytometry (Flow) |
Neat |
Control (Ctrl) |
Assay-Dependent |
| Product Specifications | |
|---|---|
Host/Isotype |
Mouse / IgG1 |
Class |
Control |
Type |
Isotype Control |
Immunogen |
Human T lymphocytes |
Conjugate |
PE |
Excitation/Emission Max |
565/576 nm
View spectra
|
Form |
Lyophilized |
Concentration |
Conc. Not Determined |
Purification |
Protein G |
Storage buffer |
PBS with 1% BSA, 5% sucrose |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2550619 |
Product Specific Information
PA5-33203 has been tested and found to be negative on the following rat tissues - peripheral blood leucocytes, thymocytes, splenocytes and macrophages.
PA5-33203 recognizes the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. This antibody is not suitable for use as a negative control on human tissues.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark. Once reconstituted, this product has a shelf life of approx. 6 months.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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