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|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||IgG gamma 1|
|Conjugate||Alexa Fluor® 546|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG2a, IgG2b and IgG3 prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||An extracellular region of Serrate is essential for ligand-induced cis-inhibition of Notch signaling.||Fleming RJ,Hori K,Sen A,Filloramo GV,Langer JM,Obar RA,Artavanis-Tsakonas S,Maharaj-Best AC||Development (Cambridge, England) (140:2039)||2013|
|Not Applicable||Not Cited||Association of TMEM16A chloride channel overexpression with airway goblet cell metaplasia.||Scudieri P,Caci E,Bruno S,Ferrera L,Schiavon M,Sondo E,Tomati V,Gianotti A,Zegarra-Moran O,Pedemonte N,Rea F,Ravazzolo R,Galietta LJ||The Journal of physiology (590:6141)||2012|
|Not Applicable||Not Cited||Different role of CD73 in leukocyte trafficking via blood and lymph vessels.||Ålgars A,Karikoski M,Yegutkin GG,Stoitzner P,Niemelä J,Salmi M,Jalkanen S||Blood (117:4387)||2011|
|Not Applicable||Not Cited||Cytokeratin 18 is a specific marker of bovine intestinal M cell.||Hondo T,Kanaya T,Takakura I,Watanabe H,Takahashi Y,Nagasawa Y,Terada S,Ohwada S,Watanabe K,Kitazawa H,Rose MT,Yamaguchi T,Aso H||American journal of physiology. Gastrointestinal and liver physiology (300:G442)||2011|
|Not Applicable||Not Cited||RbAp48 regulates cytoskeletal organization and morphology by increasing K-Ras activity and signaling through mitogen-activated protein kinase.||Scuto A,Zhang H,Zhao H,Rivera M,Yeatman TJ,Jove R,Torres-Roca JF||Cancer research (67:10317)||2007|