Immunofluorescence analysis of Goat anti-Mouse IgG1 Secondary Antibody, Alexa Fluor 546 was performed using Hep G2 cells stained with Apolipoprotein A1 (513) Mouse Monoclonal Primary Antibody (MIA1404). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG1 Secondary Antibody, Alexa Fluor 546 (A21123) was used at a concentration of 4µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of Apolipoprotein A1 in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||IgG gamma 1|
|Conjugate||Alexa Fluor® 546|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG2a, IgG2b and IgG3 prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:500|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb.
A-21123 was used in immunohistochemistry to analyze glomerular glutamatergic neurons and their control of sensory information transfer in the mouse olfactory bulb
|Tatti R,Bhaukaurally K,Gschwend O,Seal RP,Edwards RH,Rodriguez I,Carleton A||Nature communications (5:null)||2014|
|Not Applicable||Not Cited||An extracellular region of Serrate is essential for ligand-induced cis-inhibition of Notch signaling.||Fleming RJ,Hori K,Sen A,Filloramo GV,Langer JM,Obar RA,Artavanis-Tsakonas S,Maharaj-Best AC||Development (Cambridge, England) (140:2039)||2013|
|Not Applicable||Not Cited||Association of TMEM16A chloride channel overexpression with airway goblet cell metaplasia.||Scudieri P,Caci E,Bruno S,Ferrera L,Schiavon M,Sondo E,Tomati V,Gianotti A,Zegarra-Moran O,Pedemonte N,Rea F,Ravazzolo R,Galietta LJ||The Journal of physiology (590:6141)||2012|
|Not Applicable||Not Cited||Different role of CD73 in leukocyte trafficking via blood and lymph vessels.||Ålgars A,Karikoski M,Yegutkin GG,Stoitzner P,Niemelä J,Salmi M,Jalkanen S||Blood (117:4387)||2011|
|Not Applicable||Not Cited||Cytokeratin 18 is a specific marker of bovine intestinal M cell.||Hondo T,Kanaya T,Takakura I,Watanabe H,Takahashi Y,Nagasawa Y,Terada S,Ohwada S,Watanabe K,Kitazawa H,Rose MT,Yamaguchi T,Aso H||American journal of physiology. Gastrointestinal and liver physiology (300:G442)||2011|
|Not Applicable||Not Cited||RbAp48 regulates cytoskeletal organization and morphology by increasing K-Ras activity and signaling through mitogen-activated protein kinase.||Scuto A,Zhang H,Zhao H,Rivera M,Yeatman TJ,Jove R,Torres-Roca JF||Cancer research (67:10317)||2007|