Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-2 OS (Lane 2). The blots were probed with Anti LKB1 Mouse Monoclonal Antibody (Product # AHO1392, 2 µg/ml) and detected by chemiluminescence using Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # 61-0220) at dilutions 1:1,000 (Fig. 1), 1:5,000 (Fig. 2) 1:10,000 and (Fig. 3). A 55 kDa band corresponding to LKB1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX),, XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.4, with 40% glycerol, 4mg/ml BSA|
|Contains||0.1% Proclin 300|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 2 publications below|
This monoclonal Rabbit anti-Mouse IgG2a reacts with mouse IgG2a and exhibits no reactivity to the other mouse IgG subclasses, IgA, IgM or light chains.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Characterization of cross protection of Swine-Origin Influenza Virus (S-OIV) H1N1 and reassortant H5N1 influenza vaccine in BALB/c mice given a single-dose vaccination.
61-0220 was used in ELISA to evaluate the cross protection effectiveness of a single dose of either the S-OIV H1N1 or the H5N1 influenza vaccine
|Lin HT,Chuang CC,Wu HL,Chu DM,Wang YC||Journal of biomedical science (20:null)||2013|
|Not Applicable||Not Cited||IL-13 is a key regulatory cytokine for Th2 cell-mediated pulmonary granuloma formation and IgE responses induced by Schistosoma mansoni eggs.||Chiaramonte MG,Schopf LR,Neben TY,Cheever AW,Donaldson DD,Wynn TA||Journal of immunology (Baltimore, Md. : 1950) (162:920)||1999|