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Cells were pulsed with BrdU (Cat. No. B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Cat. No. A21300) and green-fluorescent Alexa Fluor® 488 Goat Anti–Mouse IgG1 isotype–specific secondary antibody (Cat. No. A21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (Cat. No. D1306, D21490). Mitochondria were labeled with anti-COX, Complex IV, subunit I, and red-fluorescent Alexa Fluor® 594 Goat Anti–Mouse IgG2a isotype-specific antibody (Cat. No. A21135). Coverslips were mounted with Prolong® antifade mounting medium (Cat. No. P7481).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG1, IgG2b and IgG3 prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 6 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||β-Catenin-SOX2 signaling regulates the fate of developing airway epithelium.||Hashimoto S,Chen H,Que J,Brockway BL,Drake JA,Snyder JC,Randell SH,Stripp BR||Journal of cell science (125:932)||2012|
|Not Applicable||Not Cited||Cytokeratin 18 is a specific marker of bovine intestinal M cell.||Hondo T,Kanaya T,Takakura I,Watanabe H,Takahashi Y,Nagasawa Y,Terada S,Ohwada S,Watanabe K,Kitazawa H,Rose MT,Yamaguchi T,Aso H||American journal of physiology. Gastrointestinal and liver physiology (300:G442)||2011|
|Not Applicable||Not Cited||Hepatitis C virus NS2 protein serves as a scaffold for virus assembly by interacting with both structural and nonstructural proteins.||Ma Y,Anantpadma M,Timpe JM,Shanmugam S,Singh SM,Lemon SM,Yi M||Journal of virology (85:86)||2011|
|Not Applicable||Not Cited||An essential role for p120-catenin in Src- and Rac1-mediated anchorage-independent cell growth.||Dohn MR,Brown MV,Reynolds AB||The Journal of cell biology (184:437)||2009|
|Not Applicable||Not Cited||Human cytomegalovirus TRS1 and IRS1 gene products block the double-stranded-RNA-activated host protein shutoff response induced by herpes simplex virus type 1 infection.||Cassady KA||Journal of virology (79:8707)||2005|
|Not Applicable||Not Cited||Dystroglycan is selectively associated with inhibitory GABAergic synapses but is dispensable for their differentiation.||Lévi S,Grady RM,Henry MD,Campbell KP,Sanes JR,Craig AM||The Journal of neuroscience : the official journal of the Society for Neuroscience (22:4274)||2002|