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Invitrogen
Mouse IgG2a Isotype Control (OX-34), FITC
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FIGURE: 1 / 1
Mouse IgG2a Isotype Control (PA5-33239) in Flow
Detection and quantification of bovine CD4+ and CD8+ T cells in Group A healthy uninfected calves using flow cytometry (FCM). (a) Bovine blood was processed and buffy coat cells were separated and counted. The cells were blocked using 4% BSA for 30 min and the staining of cells using a specific antibody. (b) Attune™ NxT Acoustic Focusing Cytometer was used for flow cytometry. Scatter plot showing gating of ... View More
Product Details
PA5-33239
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Isotype Control
Clone
OX-34
Immunogen
Activated rat T-helper cells
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-33239 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a in human tissue. This antibody may not be suitable for intracellular staining on some cell types.
The antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that PA5-33239 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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