|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Conjugate||Alexa Fluor® 350|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG1, IgG2a and IgG3 prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The impact of ageing, physical activity, and pre-frailty on skeletal muscle phenotype, mitochondrial content, and intramyocellular lipids in men.
A-21140 was used in immunohistochemistry to study the effects of ageing, physical activity, pre-frailty on skeletal muscle phenotype and examine the mitochondrial and intramyocellular lipid content of men
|St-Jean-Pelletier F,Pion CH,Leduc-Gaudet JP,Sgarioto N,Zovilé I,Barbat-Artigas S,Reynaud O,Alkaterji F,Lemieux FC,Grenon A,Gaudreau P,Hepple RT,Chevalier S,Belanger M,Morais JA,Aubertin-Leheudre M,Gouspillou G||Journal of cachexia, sarcopenia and muscle (8:213)||2017|
Failed reinnervation in aging skeletal muscle.
A-21140 was used in immunohistochemistry - frozen section to discuss the age-related presence of denervated myofibers and accelerated muscle atrophy
|Aare S,Spendiff S,Vuda M,Elkrief D,Perez A,Wu Q,Mayaki D,Hussain SN,Hettwer S,Hepple RT||Skeletal muscle (6:null)||2016|
|Not Applicable||Not Cited||
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
A-21140 was used in immunohistochemistry - frozen section to study slow-twitch type 1 muscle fibers and diaphragm assessment in mice overexpressing phospholamban
|Fajardo VA,Smith IC,Bombardier E,Chambers PJ,Quadrilatero J,Tupling AR||Brain and behavior (6:null)||2016|
Reduction in single muscle fiber rate of force development with aging is not attenuated in world class older masters athletes.
A-21140 was used in immunohistochemistry - frozen section to investigate the effects of age on the resilience of slow type fibers
|Power GA,Minozzo FC,Spendiff S,Filion ME,Konokhova Y,Purves-Smith MF,Pion C,Aubertin-Leheudre M,Morais JA,Herzog W,Hepple RT,Taivassalo T,Rassier DE||American journal of physiology. Cell physiology (310:C318)||2016|