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Invitrogen
Mouse IgG2b Isotype Control (MPC-11), DyLight™ 488
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FIGURE: 1 / 2
Mouse IgG2b Isotype Control (MA1-192-D488) in Flow
Flow cytometry analysis of CD45RA on Jurkat cells. Cells were fixed, permeabilized, and stained with a CD45RA monoclonal antibody (Product # MA1-19113, blue histogram) conjugated to DyLight 488, or a DyLight 488-conjugated mouse IgG2b isotype control (Product # MA1-192-D488, red histogram), at a concentration of 2 µg/mL. A representative 10,000 cells were acquired for each sample.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-192-D488
Applications
Tested Dilution
Western Blot (WB)
Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
Immunohistochemistry (Frozen) (IHC (F))
Assay-dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Isotype Control
Clone
MPC-11
Immunogen
KLH-coupled trinitrophenol
Conjugate
DyLight™ 488
DyLight™ 488
DyLight™ 488
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
MA1-192-D488 can be used as a negative control in any immunoassay where a DyLight 488-conjugated primary antibody of the mouse IgG2b isotype is used. DyLight 488 has an excitation/emission of 493/518nm.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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