Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution |
---|---|
Flow Cytometry (Flow) |
5 µL (1 µg)/test |
Control (Ctrl) |
Assay-Dependent |
Product Specifications | |
---|---|
Host/Isotype |
Mouse / IgG2b, kappa |
Class |
Control |
Type |
Isotype Control |
Clone |
eBMG2b |
Conjugate |
PE
|
Excitation/Emission Max |
565/576 nm View spectra |
Form |
Liquid |
Concentration |
5 µL/Test |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 0.2% BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_1518771 |
Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.
Applications Tested: This Mouse IgG2b K Isotype Control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
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