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Invitrogen
Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 570, eBioscience™
Product Details
41-4732-82
| Applications | Tested Dilution |
|---|---|
Immunohistochemistry (Paraffin) (IHC (P)) |
1 µg/mL |
Immunohistochemistry (Frozen) (IHC (F)) |
Assay-Dependent |
Immunocytochemistry (ICC/IF) |
Assay-Dependent |
Control (Ctrl) |
Assay-Dependent |
| Product Specifications | |
|---|---|
Host/Isotype |
Mouse / IgG2b, kappa |
Class |
Control |
Type |
Isotype Control |
Clone |
eBMG2b |
Conjugate |
eFluor™ 570
|
Excitation/Emission Max |
556/569 nm
View spectra
|
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2637335 |
Product Specific Information
Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.
Applications Reported: This eBMG2b antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This eBMG2b antibody has been tested by immunocytochemistry on fixed and permeabilized cells. Use isotype control at same concentration as experimental antibody. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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