|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Goats were immunized with highly purified IgG 2c isolated from C57BL/6 mice, in Freund's adjuvant.|
|Storage buffer||HEPES buffered saline, pH 7.0, with 0.2% BSA, 0.1M NaCl|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29288 detects Mouse IgG2c from mouse samples.
PA1-29288 has been successfully used in Western blot and ELISA applications.
The PA1-29288 immunogen is: Goats were immunized with highly purified IgG 2c isolated from C57BL/6 mice, in Freunds adjuvant.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.