|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.4, with 4mg/ml BSA, 40% glycerol|
|Contains||0.1% Proclin 300|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2,000-1:4,000|
|Western Blot (WB)||1:2,000-1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination.
62-6820 was used in ELISA to discuss methods to improve needle-free intradermal injections
|Arunachalam PS,Mishra R,Badarinath K,Selvam D,Payeli SK,Stout RR,Ranga U||Scientific reports (6:null)||2016|
Conditional Sox9 ablation improves locomotor recovery after spinal cord injury by increasing reactive sprouting.
62-6820 was used in western blot to examine the role of SOX9 in spinal cord injury
|McKillop WM,York EM,Rubinger L,Liu T,Ossowski NM,Xu K,Hryciw T,Brown A||Experimental neurology (283:1)||2016|
Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle.
62-6820 was used in western blot to compare calcium uptake of the sarcoplasmic reticulum between type I and type II fibers of vastus lateralis muscle of young healthy adults
|Lamboley CR,Murphy RM,McKenna MJ,Lamb GD||The Journal of physiology (592:1381)||2014|
Endogenous and maximal sarcoplasmic reticulum calcium content and calsequestrin expression in type I and type II human skeletal muscle fibres.
62-6820 was used in western blot to ascertain the relationship between sarcoplasmic reticulum calcium content and calsequestrin isoforms in human skeletal muscle
|Lamboley CR,Murphy RM,McKenna MJ,Lamb GD||The Journal of physiology (591:6053)||2013|
|Not Applicable||Not Cited||
Conditional Sox9 ablation reduces chondroitin sulfate proteoglycan levels and improves motor function following spinal cord injury.
62-6820 was used in western blot to propose SOX9 inhibition as a treatment for spinal cord injury
|McKillop WM,Dragan M,Schedl A,Brown A||Glia (61:164)||2013|