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Immunofluorescence analysis of Goat anti-Mouse IgM Heavy Chain Secondary Antibody, Alexa Fluor 568 conjugate was performed using MCF-7 cells stained withHSP70 (MB-H1) Mouse Monoclonal Primary Antibody (333800). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgM Heavy Chain Secondary Antibody, Alexa Fluor 568 conjugate (A21043) was used at a concentration of 0.2µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of HSP70 in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse Mu immunonglobin|
|Conjugate||Alexa Fluor® 568|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgGl, IgG2a, IgG2b, IgG3, IgA, human serum and purified human paraproteins prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate.
A-21043 was used in immunocytochemistry to analyze catalysis of 9-O-Acetylation of sialic acids by CASD1 through a covalent acetyl-enzyme intermediate
|Baumann AM,Bakkers MJ,Buettner FF,Hartmann M,Grove M,Langereis MA,de Groot RJ,Mühlenhoff M||Nature communications (6:null)||2015|
|Not Applicable||Not Cited||Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture.||Lahti AL,Kujala VJ,Chapman H,Koivisto AP,Pekkanen-Mattila M,Kerkelä E,Hyttinen J,Kontula K,Swan H,Conklin BR,Yamanaka S,Silvennoinen O,Aalto-Setälä K||Disease models and mechanisms (5:220)||2012|
|Not Applicable||Not Cited||Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.||Oda Y,Yoshimura Y,Ohnishi H,Tadokoro M,Katsube Y,Sasao M,Kubo Y,Hattori K,Saito S,Horimoto K,Yuba S,Ohgushi H||The Journal of biological chemistry (285:29270)||2010|
|Not Applicable||Not Cited||Cyclosporin A has direct effects on adult neural precursor cells.||Hunt J,Cheng A,Hoyles A,Jervis E,Morshead CM||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:2888)||2010|
|Not Applicable||Not Cited||Two distinct T cell subsets, CD4+ and CD8+CD60+, and their cytokines are required for in vitro induction of human ragweed-specific memory IgE responses.||Smith-Norowitz TA,Silverberg J,Norowitz KB,Bluth MH,Chice S,Joks R,Nowakowski M,Durkin HG||Journal of immunology (Baltimore, Md. : 1950) (181:4761)||2008|
|Not Applicable||Not Cited||Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures.||Benediktsson AM,Schachtele SJ,Green SH,Dailey ME||Journal of neuroscience methods (141:41)||2005|
|Not Applicable||Not Cited||Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells.||Fuja TJ,Schwartz PH,Darcy D,Bryant PJ||Journal of neuroscience research (75:782)||2004|
|Not Applicable||Not Cited||Regulation of lymphocyte apoptosis by interferon regulatory factor 4 (IRF-4).||Fanzo JC,Hu CM,Jang SY,Pernis AB||The Journal of experimental medicine (197:303)||2003|