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|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse Mu immunonglobin|
|Conjugate||Alexa Fluor® 633|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgGl, IgG2a, IgG2b, IgG3, IgA, human serum and purified human paraproteins prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Structural basis of phosphoinositide binding to kindlin-2 protein pleckstrin homology domain in regulating integrin activation.||Liu J,Fukuda K,Xu Z,Ma YQ,Hirbawi J,Mao X,Wu C,Plow EF,Qin J||The Journal of biological chemistry (286:43334)||2011|
|Not Applicable||Not Cited||A rapid, cost-effective method for counting human embryonic stem cell numbers as clumps.||Prowse A,Wolvetang E,Gray P||BioTechniques (47:599)||2009|
|Not Applicable||Not Cited||Migfilin, a molecular switch in regulation of integrin activation.||Ithychanda SS,Das M,Ma YQ,Ding K,Wang X,Gupta S,Wu C,Plow EF,Qin J||The Journal of biological chemistry (284:4713)||2009|
|Not Applicable||Not Cited||Lack of myostatin results in excessive muscle growth but impaired force generation.||Amthor H,Macharia R,Navarrete R,Schuelke M,Brown SC,Otto A,Voit T,Muntoni F,Vrbóva G,Partridge T,Zammit P,Bunger L,Patel K||Proceedings of the National Academy of Sciences of the United States of America (104:1835)||2007|
|Not Applicable||Not Cited||Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells.||Yoon KH,Yoon M,Moir RD,Khuon S,Flitney FW,Goldman RD||The Journal of cell biology (153:503)||2001|