Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and DU-145 (Lane 2). The blots were probed with Anti-STRO-1 Mouse Monoclonal Antibody (Product # 39-8401, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgM Heavy Chain Cross-Adsorbed Secondary Antibody, Biotin (Product # M32315) at dilutions 1:5,000 (Fig. 1), 1:20,000 (Fig. 2) and 1:50,000 (Fig. 3). A 75 kDa band corresponding to STRO-1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse Mu immunonglobin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against human IgG prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5,000-1:50,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
For this goat anti-mouse IgM (Heavy chain) antibody, it is reccommended to use a 1:10,000 working dilution for optimal performance.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
An orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus.
M31515 was used in immunohistochemistry - frozen section to test if an inhibitor of spleen tyrosine kinase-dependent signaling modulates disease in a mouse model of lupus via inhibition of Fc receptor and B cell receptor signaling
|Bahjat FR,Pine PR,Reitsma A,Cassafer G,Baluom M,Grillo S,Chang B,Zhao FF,Payan DG,Grossbard EB,Daikh DI||Arthritis and rheumatism (58:1433)||2008|