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Invitrogen
Mouse IgM Isotype Control, FITC
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Product Details
PA5-33229
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgM
Class
Control
Type
Isotype Control
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
PA5-33229 can be used as a negative control in flow cytometry when using mouse monoclonal antibodies in human cell surface antigens of isotype IgM. This enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components. This antibody recognizes a rat cell surface marker and therefore cannot be used as a negative control in the species.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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