Flow cytometry analysis of TRA-1-60 was performed on NCCIT cells. NCCIT cells were harvested, fixed and washed with PBS. Cells were incubated with 0.4 µg of TRA-1-60 monoclonal antibody (Product # MA1-023), followed by incubation with FITC-conjugated goat-anti-mouse IgM secondary antibody (Product # 31992), indicated by the shifted peak (blue line). To control for non-specific binding, cells were also incubated with secondary antibody alone (black line). Incubations were performed for 30 mins in the dark and 10,000 cells were analyzed for each sample.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50 - 1:200|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:50 - 1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31992 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31992 reacts with the heavy chain of mouse IgM, but does not react against IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. The concentration of antibody and buffer salts will decrease to one-half of the original after addition of glycerol. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: ~ 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 1.5 ml of distilled water (1.5 mg/ml after restoration). Product is stable for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
Country of Origin: USA
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.