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Sandwich ELISA analysis of mouse IL-1 beta was performed using a Mouse IL-1 beta Colorimetric ELISA kit (Product # EM2IL1B) by loading 50 ul per well of Mouse Interleukin-1 beta Recombinant Protein (Product # SMIL1B) in dodecuplicate at 2000, 1000, 500, 250, 125, 62, and 31 pg/ml across an anti-mouse IL-1 beta pre-coated plate and incubating for 2 hours at room temperature. The plate was washed, then incubated with 100 ul per well of an anti-mouse IL-1 beta biotinylated antibody at a concentration of 0.0023 ug/ml for 1 hour at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) in all test wells at 1:5.5 dilution for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Not Applicable|
|Host / Isotype||E.coli|
|Storage buffer||4% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
International reference standard is not available. Calibrated to a Thermo reference standard.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
4000 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.