Sandwich ELISA analysis of mouse IL-2 was performed using the a Mouse IL-2 Colorimetric ELISA Kit (Product # EMIL2) by loading 50 ul per well of a Mouse IL-2 monoclonal antibody (Product # MM100) in quadruplicate at 0 (blue line), 50% (2.5 ug/ml, red line), and 100% (5 ug/ml, green line) displacement in a plate and then loading 50 ul per well of Mouse IL-2 Recombinant Protein (Product # SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/ml across a 5 ug/ml Mouse IL-2 monoclonal antibody (Product # MM101) pre-coated plate and incubating for 2 hours at 37°C. The plate was washed, then incubated with 100 ul per well of an HRP-conjugated rat anti-mouse IL-2 antibody at a 1:25,000 dilution for 1 hour at 37°C. Detection was performed using TMB substrate solution for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Not Applicable|
|Host / Isotype||Yeast|
|Storage buffer||4% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
International reference standard is not available. Calibrated to a Thermo reference standard.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
850 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.