Sandwich ELISA analysis of mouse IL-4 was performed using a Mouse IL-4 Colorimetric ELISA kit (Product # EMIL4) by loading 50 ul per well of Mouse IL-4 Recombinant Protein (Product # SMIL4) in dodecuplicate at 375, 75, 15 and 0 pg/ml across a 2 ug/ml rat anti-mouse IL-4 (Product # MM450C) pre-coated plate and incubating for 2 hours at 37°C. The plate was washed, then incubated with 100 ul per well of an HRP-conjugated rat anti-mouse IL-4 antibody at a concentration of 0.4 ug/ml for 1 hour at 37°C. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Mouse|
|Host / Isotype||Yeast|
|Storage buffer||4% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This Mouse IL-4 Standard is calibrated against NIBSC Mouse IL-4 Reference Standard lot #91/656. 1 Thermo pg = 0.42 NIBSC pg = 0.0042 NIBSC unit
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
375 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.