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Sandwich ELISA analysis of mouse IL-6 was performed using the a Mouse IL-6 Colorimetric ELISA Kit (Product # EM2IL6) by loading 50 ul per well of Mouse IL-6 Recombinant Protein (Product # SMIL6) in dodecuplicate at 2000, 800, 320, 128, 51, and 0 pg/ml across a 3 ug/ml IL-6 monoclonal antibody (Product # MM600D) pre-coated plate and incubating for 2 hours at room temperature. The plate was washed, then incubated with 50 ul per well of a biotinylated-anti-IL-6 monoclonal antibody (Product # MM600CB) in dodecuplicate at 0.25 ug/ml for 1 hour at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) at a dilution of 1:100,000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB-ELISA substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Not Applicable|
|Published species reactivity||Mouse|
|Host / Isotype||unidentified baculovirus|
|Storage buffer||4% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 2 publications below|
The International reference standard is not available. Calibrated to a Thermo reference standard.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at 2-8°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
5000 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of regulatory T cells and prolongation of survival of fully allogeneic cardiac grafts by administration of Tokishakuyaku-san in mice.
SMIL6 was used in ELISA to investigate the effect of Japanese herbal medicine Tokishakuyaku-san on the induction of regulatory T cells and on the survival of fully allogeneic cardiac grafts in mice
|Zhang Q,Uchiyama M,Jin X,Iwami D,Shirasugi N,Watanabe T,Niimi M||Surgery (150:923)||2011|
Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes.
SMIL6 was used in ELISA to evaluate the immune responses against nanoliposomes containing immunostimulatory CpG oligodeoxynucleotide
|Erikçi E,Gursel M,Gürsel I||Biomaterials (32:1715)||2011|