Reconstitution: Sterile 10mM Acetic Acid at 0.1 mg/mL. Centrifuge vial before opening. Do not vortex. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeated freeze thaws
Storage: Store at -20°C to -80°C for 12 months, 1 month at 4°C after reconstituted or 3 months when stored at -20°C to -80°C after reconstituting as directed.
Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
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Protein Aliases: D factor; Differentiation-stimulating factor; Leukemia inhibitory factor; LIF; myeloid leukaemia inhibitory factor
Gene Aliases: Lif
UniProt ID: (Mouse) P09056
Entrez Gene ID: (Mouse) 16878