Recombinant mouse EGF is a non-glycosylated protein, containing 53 amino acids, including 3 intra-molecular disulfide-bonds, with a molecular weight of 6 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug.
The expected biological activity (ED50) is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 0.1 ng/mL. The specific activity of this protein is 1x10^7 units/mg.
Reconstitute using sterile water at 0.1 mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability.
Amino acid sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR.
Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several variants, one being VEGF-120. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: M-VEGF-121; vascular endothelial growth factor 165b; Vascular endothelial growth factor A; Vascular permeability factor; VEGF-A; VPF
Gene Aliases: Vegf; Vegfa; Vpf
UniProt ID: (Mouse) Q00731
Entrez Gene ID: (Mouse) 22339