Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), Caco-2 (Lane 2) and A-431 (Lane 3). The blot was probed with Anti-MPS1 Mouse Monoclonal Antibody (Product # 35-9100, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A ~96 kDa band corresponding to MPS1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by overnight transfer method. The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product# SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Tag, Rat, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||His6-tagged human Mps1 recombinant protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).||Naud S,Westwood IM,Faisal A,Sheldrake P,Bavetsias V,Atrash B,Cheung KM,Liu M,Hayes A,Schmitt J,Wood A,Choi V,Boxall K,Mak G,Gurden M,Valenti M,de Haven Brandon A,Henley A,Baker R,McAndrew C,Matijssen B,Burke R,Hoelder S,Eccles SA,Raynaud FI,Linardopoulos S,van Montfort RL,Blagg J||Journal of medicinal chemistry (56:10045)||2013|
|Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).||Naud S,Westwood IM,Faisal A,Sheldrake P,Bavetsias V,Atrash B,Cheung KM,Liu M,Hayes A,Schmitt J,Wood A,Choi V,Boxall K,Mak G,Gurden M,Valenti M,de Haven Brandon A,Henley A,Baker R,McAndrew C,Matijssen B,Burke R,Hoelder S,Eccles SA,Raynaud FI,Linardopoulos S,van Montfort RL,Blagg J||Journal of medicinal chemistry (56:10045)||2013|
High frequency of TTK mutations in microsatellite-unstable colorectal cancer and evaluation of their effect on spindle assembly checkpoint.
35-9100 was used in immunohistochemistry - paraffin section to characterize nonsense-mediated decay-escaping frameshift mutations in microsatellite-unstable colorectal cancers.
|Niittymäki I,Gylfe A,Laine L,Laakso M,Lehtonen HJ,Kondelin J,Tolvanen J,Nousiainen K,Pouwels J,Järvinen H,Nuorva K,Mecklin JP,Mäkinen M,Ristimäki A,Ørntoft TF,Hautaniemi S,Karhu A,Kallio MJ,Aaltonen LA||Carcinogenesis (32:305)||2011|
|Human||Not Cited||Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression.||Fisk HA,Mattison CP,Winey M||Proceedings of the National Academy of Sciences of the United States of America (100:14875)||2003|