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Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Breast Carcinoma tissue using PA5-19564, anti-Mre11 antibody. Primary antibody was used at a concentration of 5 ug/ml and exposed for 8 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Mre11.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19564 targets Mre11 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-19564 immunogen is synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Mre11.
PA5-19564 detects Mre11 which has a predicted molecular weight of approximately 81 kDa.
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AT-like disease; ATLD, HNGS1, MRE11, MRE11B, AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11-like protein A
ATLD; HNGS1; MRE11; MRE11A; MRE11B