Immunofluorescent analysis of Mu-Calpain in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mu-Calpain monoclonal antibody (Product # MA3-940) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mu-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. This image was taken using unpurified ascites antibody.
|Tested species reactivity||Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Yeast, Rabbit, Pig, Rat, Sheep, Bovine, Arthropod, Hamster, Fish, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified bovine skeletal muscle 80 kDa mu-calpain subunit.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-940 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
MA3-940 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects an 80 kDa protein representing mu-calpain from human platelets and erythrocytes and HeLa and A431 cell lysates. Immunocytochemical staining of mu-calpain in LLC-PK1 cells with MA3-940 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-940 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit. This antibody recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases.
Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Forage legumes rich in condensed tannins may increase n-3 fatty acid levels and sensory quality of lamb meat.
MA3-940 was used in western blot to study the effects of tannins on meat quality
|Girard M,Dohme-Meier F,Silacci P,Ampuero Kragten S,Kreuzer M,Bee G||Journal of the science of food and agriculture (96:1923)||2016|
Differences in calpain system, desmin degradation and water holding capacity between commercial Meishan and Duroc¿×¿Landrace¿×¿Yorkshire crossbred pork.
MA3-940 was used in western blot to investigate the differences in calpain system, desmin degradation, pH values and water holding capacity between muscles of commercial Meishan and Duroc? x ?Landrace? x ?Yorkshire crossbred pigs
|Wang J,Yan XL,Liu R,Fu QQ,Zhou GH,Zhang WG||Animal science journal = Nihon chikusan Gakkaiho (87:109)||2016|
Effect of early postmortem enhancement of calcium lactate/phosphate on quality attributes of beef round muscles under different packaging systems.
MA3-940 was used in western blot to determine the effect of calcium lactate/phosphate enhancement on the quality of beef round cuts in high-oxygen modified atmosphere
|Cruzen SM,Kim YH,Lonergan SM,Grubbs JK,Fritchen AN,Huff-Lonergan E||Meat science (101:63)||2015|
The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins.
MA3-940 was used in western blot to study the role of small heat shock proteins in protecting bovine skeletal myofibrils from post-mortem proteolytic degradation
|Lomiwes D,Hurst SM,Dobbie P,Frost DA,Hurst RD,Young OA,Farouk MM||Meat science (97:548)||2014|
Effect of nitric oxide on ¿-calpain activation, protein proteolysis, and protein oxidation of pork during post-mortem aging.
MA3-940 was used in western blot to investigate the effect of nitric oxide (NO) on calpain activation, protein proteolysis, and oxidation in porcine muscles
|Li YP,Liu R,Zhang WG,Fu QQ,Liu N,Zhou GH||Journal of agricultural and food chemistry (62:5972)||2014|
Influence of supplemental vitamin C on postmortem protein degradation and fatty acid profiles of the longissimus thoracis of steers fed varying concentrations of dietary sulfur.
MA3-940 was used in western blot to study postmortem muscle proteolysis and fatty acid profiles in beef cattle receiving different amounts of dietary sulfur and the effects of supplemental vitamin C
|Pogge DJ,Lonergan SM,Hansen SL||Meat science (96:956)||2014|
The development of meat tenderness is likely to be compartmentalised by ultimate pH.
MA3-940 was used in western blot to study the effects of ultimate pH on the development of meat tenderness in Bulls
|Lomiwes D,Farouk MM,Wu G,Young OA||Meat science (96:646)||2014|
Distinct roles for ¿-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration.
MA3-940 was used in western blot to study the distinct roles of different calpain isoforms in synaptic neuroprotection and extrasynaptic neurodegeneration mediated by the NMDA receptor
|Wang Y,Briz V,Chishti A,Bi X,Baudry M||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:18880)||2013|
Small heat shock proteins and toughness in intermediate pHu beef.
MA3-940 was used in western blot to study the role of small heat shock proteins in toughness of beef with an intermediate ultimate pH
|Lomiwes D,Farouk MM,Frost DA,Dobbie PM,Young OA||Meat science (95:472)||2013|
Pre rigor processing, ageing and freezing on tenderness and colour stability of lamb loins.
MA3-940 was used in western blot to study the effect of different aging and freezing protocols on lamb loin tenderness and color stability
|Kim YH,Luc G,Rosenvold K||Meat science (95:412)||2013|
Evaluation of feedlot cattle working chute behavior relative to temperament, tenderness, and postmortem proteolysis.
MA3-940 was used in western blot to study whether calpain activation and post-mortem proteolysis play any role in the relationship between cattle feed chute behaviour and meat tenderness
|Magolski JD,Berg EP,Hall NL,Anderson VL,Keller WL,Jeske TM,Carlin KR||Meat science (95:92)||2013|
Evidence of decreased muscle protein turnover in gilts selected for low residual feed intake.
MA3-940 was used in western blot to study the rate of protein turnover in finisher pigs receiving low residual feed intake
|Cruzen SM,Harris AJ,Hollinger K,Punt RM,Grubbs JK,Selsby JT,Dekkers JC,Gabler NK,Lonergan SM,Huff-Lonergan E||Journal of animal science (91:4007)||2013|
Profile of biochemical traits influencing tenderness of muscles from the beef round.
MA3-940 was used in western blot to study the biochemical factors governing commercially important beef cut tenderness
|Anderson MJ,Lonergan SM,Fedler CA,Prusa KJ,Binning JM,Huff-Lonergan E||Meat science (91:247)||2012|
High pre rigor temperature limits the ageing potential of beef that is not completely overcome by electrical stimulation and muscle restraining.
MA3-940 was used in western blot to study the effects on beef quality of electrical input, wrapping, pre rigor temperature and different post rigor chilling rates
|Kim YH,Stuart A,Nygaard G,Rosenvold K||Meat science (91:62)||2012|
Involvement of calpain 2 in ionomycin-induced cell death in cultured mouse lens epithelial cells.
MA3-940 was used in western blot to investigate the role of calpain 2 in the ionomycin-induced cell death in mouse lens epithelial cells
|Nakajima T,Shearer TR,Azuma M||Current eye research (36:930)||2011|
Protein denaturing conditions in beef deep semimembranosus muscle results in limited ¿-calpain activation and protein degradation.
MA3-940 was used in western blot to investigate the effect of protein denaturation on u-calpain activation
|Kim YH,Lonergan SM,Huff-Lonergan E||Meat science (86:883)||2010|
High-oxygen modified atmosphere packaging system induces lipid and myoglobin oxidation and protein polymerization.
MA3-940 was used in western blot to investigate the effect of the high-oxygen modified atmosphere packaging system on oxidation and polymerization
|Kim YH,Huff-Lonergan E,Sebranek JG,Lonergan SM||Meat science (85:759)||2010|
Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans.
MA3-940 was used in western blot to investigate the role of Arl13b in ciliary protein transport in C. elegans
|Cevik S,Hori Y,Kaplan OI,Kida K,Toivenon T,Foley-Fisher C,Cottell D,Katada T,Kontani K,Blacque OE||The Journal of cell biology (188:953)||2010|
Protease activity in post-mortem red swamp crayfish (Procambarus clarkii) muscle stored in modified atmosphere packaging.
MA3-940 was used in western blot to study the involvement of protease activity in storage of red swamp crayfish
|Chen G,Guttmann RP,Xiong YL,Webster CD,Romaire RP||Journal of agricultural and food chemistry (56:8658)||2008|
Role of mu-calpain in human decidua for recurrent miscarriage.
MA3-940 was used in western blot to study the role of mu-calpain in decidua from patients with recurrent miscarriage
|Kumagai K,Ozaki Y,Nakanishi T,Inomata M,Furuno T,Nakanishi M,Ogasawara MS||American journal of reproductive immunology (New York, N.Y. : 1989) (59:339)||2008|
Isolation and characterization of mu-calpain, m-calpain, and calpastatin from postmortem muscle. I. Initial steps.
MA3-940 was used in western blot to isolate and characterize calpain isoforms and calpastatin
|Camou JP,Mares SW,Marchello JA,Vazquez R,Taylor M,Thompson VF,Goll DE||Journal of animal science (85:3400)||2007|
Effect of postmortem storage on activity of mu- and m-calpain in five bovine muscles.
MA3-940 was used in western blot to evaluate the effect of the postmortem storage time and temperature on calpain activity in bovine muscles
|Camou JP,Marchello JA,Thompson VF,Mares SW,Goll DE||Journal of animal science (85:2670)||2007|
Rate and extent of pH decline affect proteolysis of cytoskeletal proteins and water-holding capacity in pork.
MA3-940 was used in western blot to study the effect of pH changes on breakdown of porcine cytoskeletal proteins
|Bee G,Anderson AL,Lonergan SM,Huff-Lonergan E||Meat science (76:359)||2007|
Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss.
MA3-940 was used in western blot to purify and characterize the calpain and calpastatin from rainbow trout
|Saito M,Li H,Thompson VF,Kunisaki N,Goll DE||Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology (146:445)||2007|
Contribution of postmortem changes of integrin, desmin and ¿-calpain to variation in water holding capacity of pork.
MA3-940 was used in western blot to investigate the postmortem changes of integrin, desmin, and calpain in pork
|Zhang WG,Lonergan SM,Gardner MA,Huff-Lonergan E||Meat science (74:578)||2006|
Effects of 25-hydroxyvitamin D3 and manipulated dietary cation-anion difference on the tenderness of beef from cull native Korean cows.
MA3-940 was used in western blot to study the effect of 25-hydroxyvitamin D3 and manipulated dietary cation-anion difference on beef quality
|Cho YM,Choi H,Hwang IH,Kim YK,Myung KH||Journal of animal science (84:1481)||2006|
Calpain may contribute to hereditary cataract formation in sheep.
MA3-940 was used in western blot to investigate the role of calpain during hereditary cataract formation in sheep
|Robertson LJ,Morton JD,Yamaguchi M,Bickerstaffe R,Shearer TR,Azuma M||Investigative ophthalmology and visual science (46:4634)||2005|
Effect of pH and ionic strength on mu- and m-calpain inhibition by calpastatin.
MA3-940 was used in western blot to study the effect of pH and ionic strength on mu- and m-calpain activity and the ability of calpastatin to inhibit the activity of mu- or m-calpain
|Maddock KR,Huff-Lonergan E,Rowe LJ,Lonergan SM||Journal of animal science (83:1370)||2005|
Oxidative environments decrease tenderization of beef steaks through inactivation of mu-calpain.
MA3-940 was used in western blot to investigate the role of inactivation of mu-calpain for tenderization of beef steaks during oxidative environments
|Rowe LJ,Maddock KR,Lonergan SM,Huff-Lonergan E||Journal of animal science (82:3254)||2004|
The calpain system in human placenta.
MA3-940 was used in western blot to study the calpains present in human placenta
|Thompson VF,Saldaña S,Cong J,Luedke DM,Goll DE||Life sciences (70:2493)||2002|
Changes in the calpains and calpastatin during postmortem storage of bovine muscle.
MA3-940 was used in western blot to detect the changes of micro-calpain, m-calpain, and calpastatin in bovine muscle during postmortem storage
|Boehm ML,Kendall TL,Thompson VF,Goll DE||Journal of animal science (76:2415)||1998|
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains.
MA3-940 was used in western blot to study the effect of specific calpain antibodies for the regulation of calpain function
|Cong J,Thompson VF,Goll DE||The Journal of biological chemistry (268:25740)||1993|
Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles.
MA3-940 was used in immunohistochemistry to investigate the effect of early postmortem biochemical factors on tenderness and water-holding capacity of three porcine muscles
|Melody JL,Lonergan SM,Rowe LJ,Huiatt TW,Mayes MS,Huff-Lonergan E||Journal of animal science (82:1195)||2004|
Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle.
MA3-940 was used in immunohistochemistry to study the importance of two major proteolytic systems in tranforming rabbit and rat muscles
|Sultan KR,Dittrich BT,Leisner E,Paul N,Pette D||American journal of physiology. Cell physiology (280:C239)||2001|
The endoplasmic reticulum chaperone glycoprotein GRP94 with Ca(2+)-binding and antiapoptotic properties is a novel proteolytic target of calpain during etoposide-induced apoptosis.
MA3-940 was used in immunocytochemistry to show that a calcium-binding ER protein with protective functions against calcium-induced apoptosis is a substrate for a calcium-activated protease
|Reddy RK,Lu J,Lee AS||The Journal of biological chemistry (274:28476)||1999|
A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells.
MA3-940 was used in immunocytochemistry to compare the cellular localization of calpains and calpastatin in human A431 cells
|Lane RD,Allan DM,Mellgren RL||Experimental cell research (203:5)||1992|