|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human milk fat globule.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06503 detects episialin in human samples.
MA1-06503 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry.
The MA1-06503 immunogen is a human milk fat globule.
Store at 4°C or in small aliquots at -20°C if preferred. Avoid freeze-thaw cycles.
Episialin is also known as MUC1, MAM-6, CA 15-3, PEM and EMA. It is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. The gene encoding episialin (MUC1) is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large
differences in molecular weight of the protein. Episialin is present in several secretory epithelial cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Mucin1 shifts Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway by activating JNK in human hepatocellular carcinoma cells.
MA1-06503 was used in flow cytometry, immunohistochemistry - paraffin section, and western blot to study the shift of Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway occurs by Mucin1 activating J
|Li Q,Liu G,Yuan H,Wang J,Guo Y,Chen T,Zhai R,Shao D,Ni W,Tai G||Oncotarget (6:4253)||2015|
Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report.
MA1-06503 was used in immunohistochemistry to describe a tumor from a patient with oncocytic cystadenoma
|Ba¿ak K,Kiro¿lu K||Medicine (93:null)||2014|
Multinodular reticular schwannoma in the head and neck region: a potential diagnostic pitfall.
MA1-06503 was used in immunohistochemistry to report on a case of multinodular reticular schwannoma in the head and neck region
|Lau PP,Yau DT,Lau WH,Mak LS,Chan JK||International journal of surgical pathology (21:54)||2013|
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
MA1-06503 was used in immunohistochemistry to examine the epithelial differentiation of human embryonic stem cells
|Metallo CM,Azarin SM,Moses LE,Ji L,de Pablo JJ,Palecek SP||Tissue engineering. Part A (16:213)||2010|
Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.
MA1-06503 was used in immunohistochemistry to report a clinical case of T-cell non-Hodgkin lymphoma with unusual inflammatory breast carcinoma similarity
|Kelten C,Kabukcu S,Sen N,Teke Z,Yaren A,Erdem E,Duzcan E||Archives of gynecology and obstetrics (280:149)||2009|
Myointimoma of the glans penis.
MA1-06503 was used in immunohistochemistry to report on a case of myointimoma of the glans penis
|Vardar E,Gunlusoy B,Arslan M,Kececi S||Pathology international (57:158)||2007|
Pulmonary large cell carcinoma with rhabdoid phenotype.
MA1-06503 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype
|Yilmazbayhan D,Ates LE,Dilege S,Gulluoglu M,Tanju S,Kalayci G||Annals of diagnostic pathology (9:223)||2005|
Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study.
MA1-06503 was used in immunohistochemistry - paraffin section to compare meningiomas and mesotheliomas immunohistochemically
|Abdelzaher E,Abdallah DM||BioMed research international (2014:null)||2014|
Expression of human full-length MUC1 inhibits the proliferation and migration of a B16 mouse melanoma cell line.
MA1-06503 was used in immunocytochemistry and western blot to study the mechanisms by which expression of full length human MUC1 by the murine B16 melanoma cell inhibits its proliferation and invasiveness
|Wang F,Li Q,Ni W,Fang F,Sun X,Xie F,Wang J,Wang F,Gao S,Tai G||Oncology reports (30:260)||2013|
Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.
MA1-06503 was used in immunocytochemistry to study the mechanism by which an anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells
|Hisatsune A,Nakayama H,Kawasaki M,Horie I,Miyata T,Isohama Y,Kim KC,Katsuki H||Biochemical and biophysical research communications (405:377)||2011|
Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells.
MA1-06503 was used in immunocytochemistry to investigate production and secretion and of mucin glycoforms in HT29-18N2 cells
|Stanley CM,Phillips TE||The American journal of physiology (277:G191)||1999|