Immunofluorescence analysis of MUC1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC1 (GP1.4) Mouse Monoclonal Antibody (MA513168) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human milk fat globule membranes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2ug/test|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13168 targets Epithelial Membrane Antigen in IHC (P), ICC/IF and FACS applications and shows reactivity with human and mouse samples.
The MA5-13168 immunogen is human milk fat globule membranes.
Epithelial membrane antigen (EMA), is a mucin-like glycoprotein. Antibody to EMA has been shown useful as a pan- epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Mucin1 shifts Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway by activating JNK in human hepatocellular carcinoma cells.
MA5-13168 was used in flow cytometry, immunohistochemistry - paraffin section, and western blot to study the shift of Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway occurs by Mucin1 activating J
|Li Q,Liu G,Yuan H,Wang J,Guo Y,Chen T,Zhai R,Shao D,Ni W,Tai G||Oncotarget (6:4253)||2015|
Expression of human full-length MUC1 inhibits the proliferation and migration of a B16 mouse melanoma cell line.
MA5-13168 was used in immunocytochemistry and western blot to study the mechanisms by which expression of full length human MUC1 by the murine B16 melanoma cell inhibits its proliferation and invasiveness
|Wang F,Li Q,Ni W,Fang F,Sun X,Xie F,Wang J,Wang F,Gao S,Tai G||Oncology reports (30:260)||2013|
Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.
MA5-13168 was used in immunocytochemistry to study the mechanism by which an anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells
|Hisatsune A,Nakayama H,Kawasaki M,Horie I,Miyata T,Isohama Y,Kim KC,Katsuki H||Biochemical and biophysical research communications (405:377)||2011|
Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells.
MA5-13168 was used in immunocytochemistry to investigate production and secretion and of mucin glycoforms in HT29-18N2 cells
|Stanley CM,Phillips TE||The American journal of physiology (277:G191)||1999|
Multinodular reticular schwannoma in the head and neck region: a potential diagnostic pitfall.
MA5-13168 was used in immunohistochemistry to report on a case of multinodular reticular schwannoma in the head and neck region
|Lau PP,Yau DT,Lau WH,Mak LS,Chan JK||International journal of surgical pathology (21:54)||2013|
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
MA5-13168 was used in immunohistochemistry to examine the epithelial differentiation of human embryonic stem cells
|Metallo CM,Azarin SM,Moses LE,Ji L,de Pablo JJ,Palecek SP||Tissue engineering. Part A (16:213)||2010|
Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.
MA5-13168 was used in immunohistochemistry to report a clinical case of T-cell non-Hodgkin lymphoma with unusual inflammatory breast carcinoma similarity
|Kelten C,Kabukcu S,Sen N,Teke Z,Yaren A,Erdem E,Duzcan E||Archives of gynecology and obstetrics (280:149)||2009|
Myointimoma of the glans penis.
MA5-13168 was used in immunohistochemistry to report on a case of myointimoma of the glans penis
|Vardar E,Gunlusoy B,Arslan M,Kececi S||Pathology international (57:158)||2007|
Pulmonary large cell carcinoma with rhabdoid phenotype.
MA5-13168 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype
|Yilmazbayhan D,Ates LE,Dilege S,Gulluoglu M,Tanju S,Kalayci G||Annals of diagnostic pathology (9:223)||2005|