Immunohistochemistry analysis of Mucin 1 / MUC-1 showing staining in the cytoplasm and membrane of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 1 / MUC-1 Rabbit Polyclonal Antibody (PA516847) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from cytoplasmic tail of human MUC-1 protein|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-16847 targets MUC-1 in IHC (P) applications and shows reactivity with Human and mouse samples.
The PA5-16847 immunogen is a synthetic peptide from cytoplasmic tail of human MUC-1 protein.
MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Generation and hepatic differentiation of human iPS cells.
PA5-16847 was used in immunohistochemistry to develop a method for the generation and hepatic differentiation of human induced pluripotent stem cells
|Ishikawa T,Hagiwara K,Ochiya T||Methods in molecular biology (Clifton, N.J.) (826:103)||2011|