Immunofluorescent analysis of Mucin 2 (green) showing staining in the cytoplasm of human small intestine tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with a Mucin 2 monoclonal antibody (Product # MA5-12345) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MUC2 tandem repeat peptide|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-12345 targets Mucin 2 in IHC (P), ICC/IF and FACS applications and shows reactivity with human and mouse samples.
The MA5-12345 immunogen is mUC2 tandem repeat peptide.
Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Early Gastric Cancer-Like Advanced Gastric Cancer versus Advanced Gastric Cancer-Like Early Gastric Cancer.
MA5-12345 was used in immunohistochemistry to study endoscopic differences between early gastric cancer that resembles advanced gastric cancer and advanced gastric cancer that resembles early gastric cancer
|Park HS,Lee SY,Hong SN,Kim JH,Sung IK,Park HS,Shim CS,Jin CJ||Clinical endoscopy (46:155)||2013|
Change of mucosal color in early gastric cancer.
MA5-12345 was used in immunohistochemistry to report on the diagnostic value of mucosal color changes in early gastric cancer
|Lee HH,Lee SY,Yoon HY,Hong SN,Kim JH,Sung IK,Park HS,Shim CS,Han HS||Journal of digestive diseases (13:510)||2012|
MUC5AC, cytokeratin 20 and HER2 expression and K-RAS mutations within mucinogenic growth in congenital pulmonary airway malformations.
MA5-12345 was used in immunohistochemistry to investigate the changes of gene expression in congenital pulmonary airway malformations
|Rossi G,Gasser B,Sartori G,Migaldi M,Costantini M,Mengoli MC,Piccioli S,Cavazza A,Rivasi F||Histopathology (60:1133)||2012|
Unclassified mucin phenotype of gastric adenocarcinoma exhibits the highest invasiveness.
MA5-12345 was used in immunohistochemistry to investigate phenotypic mucin expression in human gastric adenocarcinoma
|Han HS,Lee SY,Lee KY,Hong SN,Kim JH,Sung IK,Park HS,Jin CJ,Min YI||Journal of gastroenterology and hepatology (24:658)||2009|