Immunohistochemistry analysis of Mucin 3 showing staining in the membrane and weak cytoplasm of paraffin-embedded human hepatocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 3 Mouse Monoclonal Antibody (MA512350) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||MUC3 tandem repeat peptide|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
MA5-12350 targets Mucin 3 in IHC (P) applications and shows reactivity with Human samples.
The MA5-12350 immunogen is mUC3 tandem repeat peptide.
Mucin 3 (MUC3) is a glycoprotein. Distributed in colon and rectum, and to a lesser extent in breast, lung and salivary gland tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MUC5AC, cytokeratin 20 and HER2 expression and K-RAS mutations within mucinogenic growth in congenital pulmonary airway malformations.
MA5-12350 was used in immunohistochemistry to investigate the changes of gene expression in congenital pulmonary airway malformations
|Rossi G,Gasser B,Sartori G,Migaldi M,Costantini M,Mengoli MC,Piccioli S,Cavazza A,Rivasi F||Histopathology (60:1133)||2012|
Development of human minor salivary glands: expression of mucins according to stage of morphogenesis.
MA5-12350 was used in immunohistochemistry to study mucin expression patterns during development of human minor salivary glands
|Teshima TH,Ianez RF,Coutinho-Camillo CM,Buim ME,Soares FA,Lourenço SV||Journal of anatomy (219:410)||2011|
Selective induction of mucin-3 by hypoxia in intestinal epithelia.
MA5-12350 was used in immunohistochemistry to investigate the effect of hypoxia on mucin 3 expression and its mechanism
|Louis NA,Hamilton KE,Canny G,Shekels LL,Ho SB,Colgan SP||Journal of cellular biochemistry (99:1616)||2006|